survivin蛋白及其mRNA在子痫前期患者胎盘中的表达.docVIP

survivin蛋白及其mRNA在子痫前期患者胎盘中的表达 作者： 作者：李雪兰，李春芳，王少丽，苟文丽【摘要】 目的 探讨survivin蛋白及其mRNA的表达与子痫前期的关系。方法 采用免疫组化SP法和实时荧光定量PCR(FQPCR)技术，检测20例轻度子痫前期、25例重度子痫前期和20例正常晚孕妇女胎盘组织中survivin蛋白及其mRNA的表达情况。结果 survivin蛋白主要位于绒毛小叶的合体滋养细胞及细胞滋养细胞，在子痫前期胎盘中的表达较正常晚孕妇女明显降低，且重度子痫前期的表达明显低于轻度;survivin mRNA在子痫前期胎盘中的表达也明显低于正常晚孕，重度明显低于轻度。结论 胎盘组织中survivin蛋白及其mRNA的异常表达可能在子痫前期的发生中起着重要的作用。 【关键词】 子痫前期;胎盘;survivin ABSTRACT: Objective To detect the expressions of survivin protein and survivin mRNA in placenta of women with preeclampsia and normal pregnancy.Methods The expressions of survivin protein and survivin mRNA collected from placenta of 45 preeclampsia patients (20 cases of mild preeclampsia and 25 cases of severe preeclampsia) and 20 normal pregnant women were determined by immunohistochemistry. The image analysis technique was employed to analyze survivin protein. Realtime FQPCR was used to detect the placental expression of survivin mRNA.Results survivin protein was found mostly in syncytiotrophoblasts and cytotrophoblasts, and it was significantly lower in placenta of preeclampsia than that in normal placenta, and significantly lower in severe preeclampsia than in mild preeclampsia. survivin mRNA was significantly lower in preeclampsia than in normal placenta and significantly lower in severe preeclampsia than in mild preeclampsia.Conclusion The abnormal expressions of survivin protein and mRNA in placental tissues may play an important role in the pathogenesis of preeclampsia. KEY WORDS: preeclampsia; placenta; survivin 子痫前期(preeclampsia)是妊娠期常见且严重威胁母婴健康的疾病。大量研究表明，子痫前期与滋养细胞的凋亡和浅着床有关，而survivin是一种抗凋亡作用较强的凋亡抑制因子。因此，本研究通过检测胎盘组织中survivin蛋白及mRNA的表达，探讨survivin与子痫前期的关系。 1 对象与方法 1.1 研究对象 选择2007年10月至2008年10月在西安交通大学医学院第一附属医院产科住院分娩的正常晚孕妇女20例，平均年龄(27.6±2.4)岁，平均孕周(37.6±2.5)周;轻度子痫前期孕妇20例，平均年龄(25.8±3.2)岁，平均孕周(36.4±1.7)周;重度子痫前期者25例，平均年龄(26.1±2.4)岁，平均孕周(35.7±2.3)周。3组间的年龄与孕周无明显差异。所有标本均于剖宫产术中采集。胎盘娩出后立即剪取脐带附着周围的母面胎盘组织(避开钙化灶及机化灶)约1cm×1cm×1cm大小两块，一块立即放入1.5mL Eppendorf管(已预先用1mL/L DEPC水浸泡过夜，灭菌水淋洗，并于100℃烤干)，于-80