卵巢子宫内膜异位症组织中PTEN基因mRNA及蛋白表达的研究.docVIP

卵巢子宫内膜异位症组织中PTEN基因mRNA及蛋白表达的研究 【摘要】目的探讨卵巢子宫内膜异位症组织中PTEN基因mRNA及蛋白的表达及其临床意义。方法应用半定量逆转录聚合酶链反应（RTPCR）检测卵巢子宫内膜异位症组织和正常卵巢组织中PTEN基因外显子1—9和5—9的mRNA表达水平，免疫组织化学方法检测其蛋白表达，同时结合病人的临床资料进行分析。结果卵巢子宫内膜异位症组织中PTEN外显子1—9、5—9 mRNA相对表达水平高于正常卵巢组织，差异有显著性（P0.01，P0.05）。卵巢子宫内膜异位症组织中PTEN mRNA表达水平与病人年龄、囊肿大小无关（P0.05）。与临床分期有关（P0.05）。卵巢子宫内膜异位症组织中Ⅰ～Ⅱ期有2例细胞核PTEN蛋白呈中等染色表达，与正常卵巢组织中PTEN蛋白的表达比较差异无显著性（P0.05）。结论PTEN mRNA及蛋白表达水平的异常可能是卵巢子宫内膜异位症发生的早期事件。 【关键词】 PTEN基因 子宫内膜异位症 卵巢 基因表达 免疫组织化学 Study on expressions of PTEN mRNA and protein in ovarian endometrioma XU Bing, ZHENG Qingmei, LIU Suqin, DAI Shuzhen (Dept. of Obstetrics and Gynecology, Medical School Hospital of Qingdao University, Qingdao 266003, China；Nursing School, Medical College of Qingdao University，Qingdao 266003, China；Dept. of Obstetrics and Gynecology, The Eighth Peoplersquo;s Hospital of Qingdao, Qingdao 266100, China) Abstract： Objective: To investigate PTEN mRNA and protein expressions in the ovarian endometrioma and its clinical significance. Methods: The expressions of PTEN mRNA and protein were examined by semiquantitative reverse transcription polymerase chain reaction (RTPCR) and immunohistochemistry respectively in the ovarian endometrioma and normal ovarian tissues. The clinical characteristics were compared and clinical significance was analyzed. Results: The mRNA expressions of exons 19,59 in PTEN gene in ovarian endometrioma tissues were significantly higher than those in normal ovarian tissues (P0.01, P0.05). The expression level of PTEN mRNA in ovarian endometriosis was related to staging(P0.01),but not to the patient age or the size of cyst(P0.05). There was no significant difference in the expression of PTEN protein between ovarian endometrioma and normal ovarian tissues (P0.05). Conclusion: The abnormal expressions of PTEN mRNA and protein may represent an early event in the genesis of ovarian endometrioma. Key words: PTEN gene; ovarian endometrioma; RTPCR; immunohistochemistry PTEN 是一种抑癌基因［1］，是第一个具有双特异性磷酸酶活性的基因。目