大剂量孕激素治疗对子宫内膜腺癌细胞凋亡和bcl2、bax蛋白表达的影响.docVIP

大剂量孕激素治疗对子宫内膜腺癌细胞凋亡和bcl2、bax蛋白表达的影响 【摘要】 目的：探讨大剂量孕激素治疗对子宫内膜腺癌组织细胞凋亡及其调控基因bcl2、bax蛋白表达的影响。方法：选取2000～2005年收住院手术治疗、资料完整的子宫内膜腺癌患者病理标本30例，应用原位细胞凋亡检测技术（TUNEL）检测孕激素治疗前后子宫内膜腺癌组织细胞凋亡指数，其中16例采用免疫组化SP法检测bcl2和bax蛋白表达。结果：孕激素治疗前后子宫内膜腺癌组织细胞凋亡指数、bcl2和bax蛋白表达率分别为(6.00±5.68)和(27.67±2.41)、87.50％和75.00％、100.00％和93.75％。孕激素治疗后子宫内膜腺癌组织中细胞凋亡指数较治疗前明显增加（P0.01），其变化与bcl2及bax蛋白表达无关。结论：大剂量孕激素治疗通过促进细胞分化、增加细胞凋亡而达到治疗子宫内膜腺癌的目的。 【关键词】 子宫内膜腺癌； 细胞凋亡； bcl2； bax　 Influence of high dose progesterone on apoptosis and bcl2, bax protein expression in patients with endometrial adenocarcinoma CHEG Jingxin, YUAN Min, CHEN Xiao, et al (Department of Gynecology, Affiliated Tumor Hospital, Xinjiang Medical University, Urumqi 830011, China) Abstract: Objective: To investigate the influence of high dose progesterone on apoptosis and bcl2, bax protein expression in patients with endometrial adenocarcinoma. Methods： Thirty patients with endometrial adenocarcinoma were received high dose progesterone treatment. Before and after treatment, the apoptotic cells were detected by the methods of terminal deoxynucleotidyl transferase nick end labeling, and 16 of them were examined bcl2 and bax protein expression by immunohistochemistry. Results： The expression rate of apoptosis index and and bcl2, bax before and after treatment were (6.00±5.68) and (27.67±2.41), 87.50％ and 75.00％, 100.00％ and 93.75％, respectively. There were remarkable increment of apoptosis index in endometrial adenocarcinoma after high dose progesterone treatment （P0.05）, and their changes are not correlated to bcl2 and bax （P0.05）. Conclusion： High dose progesterone treatment may play an important role in inducing cellular differentiation and apoptosis of endometrial adenocarcinoma tissues. Key words: endometrial adenocarcinoma; apoptosis; bcl2; bax 子宫内膜癌是最常见的女性生殖系统恶性肿瘤之一，其发病率逐年呈上升趋势。近年来的研究发现，细胞凋亡程序的启动与关闭控制着子宫内膜的周期性变化，细胞凋亡失常是子宫内膜癌发生的重要病理基础［1］,而bcl2家族成员在子宫内膜组织中有组织特异性［2］。本研究应用原位细胞凋亡检测技术(TUNEL)检测30例子宫内膜腺癌患者大剂量孕激素治疗前后子宫内膜腺癌组织细胞凋亡指数及其凋亡调控基因bcl2