大鼠胎血间充质干细胞移植对局灶性脑缺血治疗作用的研究.docVIP

大鼠胎血间充质干细胞移植对局灶性脑缺血治疗作用的研究 【摘要】目的：研究大鼠胎血间充质干细胞(MSCs)移植入大脑中动脉栓塞(MCAO)大鼠模型后大鼠神经功能的恢复情况。方法:线栓法建立左侧大鼠MCAO模型,随机分为3组:手术组、MSCs 移植组、生理盐水组。5溴2脱氧尿核苷(BrdU) 标记的MSCs移植后，采用改良神经功能损伤评分系统(mNSS)评价大鼠神经功能恢复情况；应用免疫组织化学双染技术检测MSCs的存活、迁移及其巢蛋白（Nestin）、胶质纤维酸性蛋白(GFAP) 和神经元特异性烯醇化酶（NSE）的表达。结果:MSCs增殖能力强，并能在体外诱导分化为成骨细胞、脂肪细胞和神经元样细胞。MSCs移植后显著提高局灶性脑缺血大鼠神经功能的恢复。移植的MSCs细胞可在大鼠脑组织中存活,并向缺血区域迁移, 有不同比例MSCs 细胞分别表达Nestin、 GFAP、NSE。结论:大鼠胎血中含有MSCs，体外扩增纯化后可促进局灶性脑缺血大鼠神经功能的恢复,移植细胞可在大鼠脑缺血区域中存活、迁移并向星形胶质细胞或神经元分化。 【关键词】 胎血 干细胞 缺氧缺血 脑 移植 大鼠 Wistar ATransplantation of fetal bloodderived mesenchymal stem cells in the treatment of cerebral ischemia in rats JU Xiuli1, HUANG Zhiwei1, WEI Xinbing2, HOU Huaishui3, SHI Qing3, LI Dong3, SUN Nianzheng1 (1. Department of Pediatrics, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China; 2. Department of Parmacology, School of Medicine, Shandong University, Jinan 250012, Shandong, China; 3.Cryomedicine Laboratory, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China) ［ABSTRACT］ Objective: To observe the role of purified mesenchymal stem cells (MSCs) derived from fetal blood on the neurological function in rats with middle cerebral artery occlusion (MCAO). Methods: Thirty adult male Wistar rats were randomly divided into three groups: the operation group, the transplantation of MSCs group and the injection of saline group. Functional outcome measurements were performed based on the modified neurological severity score(mNSS) at day 1, 7, 14, 21 and 28 after MACO. The survival, migration, expression of Nestin, glial fibriliary acidic protein (GFAP) and neuronspecific enolase(NSE) of 5bromo2deoxyuridine（BrdU）labeled MSCs were detected by immunohistochemical double staining. Results: MSCs from the fetal blood had a powerful proliferation ability. MSCs significantly improved the neurological function (P0.05). The engrafted cells survived and migrated to the ischemic tissues. (2.88±0.76) %， (5.24±1.06)% and (1.34±1.29)% of the MSCs respect