急性脊髓损伤大鼠mir-223对中性粒细胞介导炎症反应相关性分析-correlation analysis of mir - 223 on neutrophil-mediated inflammatory response in rats with acute spinal cord injury.docx

低，可以减轻中性粒细胞介导的炎症反应。【关键词】:急性脊髓损伤、miR-223、IL-6、IGF1-R、炎症、继发性损伤AcutespinalcordinjuryinratsofmiR-223neutrophilsmediatedinflammatoryresponsecorrelationstudies【Abstract】ThepurposeofthisstudywastodetecttheacutespinalcordinjuryinratsinthechangesofinflammatoryfactorsandmiR-223expression,andexpressionofmiR-223anditsdownstreamgeneIGF1-R,analysisofmiR-223andneutrophilsmediatedinflammatoryreactionsinspinalcordsecondaryinjuryprocess,andtodiscussthepossibilityasarelievesecondaryspinalcordinjury.【Methods】ThemodifiedAllen,establishesamodelofacutespinalcordinjuryinrats.The198ratswererandomlydividedintogroupswithoutspinalcordinjury(GroupA,Controlgroup,18rats),mildacutespinalcordinjury(GroupB,thedamageheight2cm),severeacutespinalcordinjury(groupC,damageheight5cm).Mildandseverespinalcordinjurygroupisdividedinto6h,12h,24h，3d,7dgroupafterinjuryand18ratsineachgroup.In6h,12h,24h,3d,7dafteroperation,carryontheBBBscoreevaluationofneuralfunction;HEdyeingtoobservethemorphologicalchanges;usingthereal-timequantitativePCR（Real-timeqPCR）toobservetheexpressionofspinalcordinjuryofratsatdifferenttimepointsafterratspinalcordinjury(miR-223,downstreamgeneIGF1-randinflammationfactors(TNF-a,IL-1,IL-6).UsinginsituhybridizationtoanalysethemiR-223andIGF1-Rspatialrelationshipinspinalcordinjurytissue.【Results】Withthedevelopmentofinflammatoryresponse,the3-6hinflammationfactorsincreasegradually,andin12-24wereatthepeakofexpression,3Ddecreasedgradually,throughthedifferentdegreesofspinalcordinjury(Milddamageheight2cm-Severeheight5cm),theresultsshowedthatMIR-223withtheexpressionofil-6contenthadtheconsistentexpressionatthatpointintime,Wheninjuryislighter,theIl-6expressionwaslow,andthemiR-223expressionwasalsolow,Whentheinjuryisheavier,theil-6expressionwashigherinmiR-223expressionwashigher,too.Thereal-timequantitativePCRtechniqueshowedthattherewascorrelationbetweenmiR-223andIL-6inspinalcordinjury.Mildorseverespinalcordinjuryafter3-6hIGF1-rexpressiongraduallyreduced,and12to24hattheminimum,graduallyrecoveredin3days,butmiR