hplc法测定兔角膜基质中核黄素含量的实验分析-experimental analysis on determination of riboflavin content in rabbit corneal stroma by hplc.docx
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hplc法测定兔角膜基质中核黄素含量的实验分析-experimental analysis on determination of riboflavin content in rabbit corneal stroma by hplc
2.3统计学处理............................................................................303结果.................................................................................................303.1各组角膜组织的变化及检测色谱曲线................................303.2各组角膜组织中核黄素的含量............................................354讨论.................................................................................................365结论....................................................................................................39参考文献...............................................................................................39附录.............................................................................................................44致谢.............................................................................................................46综述.............................................................................................................47跨角膜上皮核黄素-紫外线胶原交联的研究进展.............................47参考文献...............................................................................................54英文缩略词表(Abbreviations)英文缩写英文全称中文全称BAKbenzalkoniumchloride苯扎氯铵CXL/C3-Rcornealcollagencross-linking角膜胶原交联DNAdeoxyribonucleicacid脱氧核糖核酸EDTAethylenediaminetetraaceticacid乙二胺四乙酸EDTANa2ethylenediaminetetraaceticaciddisodiumsalt乙二胺四乙酸二钠FADflavinadeninedinucletide黄素腺嘌呤二核苷酸FMNflavinmononucleotide黄素单核苷酸HPLChighperformanceliquidchromatograph高效液相色谱IL-1interleukin-1白细胞介素-1MMP-2matrixmetalloproteinase-2基质金属蛋白酶-2MT1-MMPmembranetargeted1-matrixmetalloprote-inase膜型1基质金属蛋白酶ROSreactiveoxygenspecies活性氧族RSDrelativestandarddeviation相对标准偏差SDstandarddeviation标准偏差TCAcrichloroaceticacid三氯乙酸TECXLtransepithelialcornealcollagencross-link-ing跨角膜上皮胶原交联TIMPtissueInhibitorofmetalloproteinase基质金属蛋白酶组织抑制因子UVAultravioletA紫外线A第一部分兔角膜基质中核黄素含量测定的HPLC方法建立中文摘要目的建立兔角膜基质中核黄素含量测定的高效液相色谱(highperformanceliquidchromatograph,HPLC)分析方法,并分析该方法的专属性、灵敏度和准确性。方法兔角膜组织匀浆经加热孵育和10%三氯乙酸去蛋白后离心,组织上清液经C18-SPE柱固相萃取后检测。高效液相色谱条件:色谱柱柱选择SinaChromODS-B
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