金纳米空心球同载反义mir-21阿霉素及光响应性控制释放研究-study on antisense mir - 21 adriamycin co-loaded with gold hollow nanospheres and its photoresponsive controlled release.docxVIP

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金纳米空心球同载反义mir-21阿霉素及光响应性控制释放研究-study on antisense mir - 21 adriamycin co-loaded with gold hollow nanospheres and its photoresponsive controlled release.docx

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金纳米空心球同载反义mir-21阿霉素及光响应性控制释放研究-study on antisense mir - 21 adriamycin co-loaded with gold hollow nanospheres and its photoresponsive controlled release

AbstractIn present thesis, a gene/drug co-loading system based on hollow gold nanospheres (HGNS) was designed and the sequential releasing of as-miR-21 and doxorubicin (DOX) response to near-infrared laser was investigated.Firstly, HGNS were prepared employing Cu2O nanoparticles and silver nanoparticles as template respectively. The morphologies and spectrum properties of HGNS were studied via transmission electron microscopy (TEM) and UV-visible spectrophotometer measurement. The results showed that, in the case of CuO2 as template, HGNS exhibited a porous structure which seem formed by the aggregation of gold nanoparticles, and an adsorption peaks at 650 nm was found. Both the amount and the method for HAuCl4 addition affected the morphologies and spectrum properties of HGNS. The shell of HGNS became more complete when the concentration of HAuCl4 increased from 0 to 0.13 mM, and a slight Red Shift was observed. Further increased the concentration (amount) of HAuCl4 increased the thickness of shell, leading to the Blue Shift of the adsorption peak. Compared with the littlie by littlie method, feeding the HAuCl4 in one batch led the shell of HGNS more complete, and the peak of spectra shifted to near infrared region. Nevertheless, no HGNS obtained by this method exhibit absorption peak in near-infrared region(800~2500 nm) where the laser can penetrate deep tissue and trigger the release of drug from HGNs.. The HGNS with adsorption peak at 960 nm were successful synthesized with silver as template. The HGNS were well dispersed with uniform size60～70nm.Secondly, PAMAM dendrimer was covalently conjugated onto the surface of HGNS, and as-miR-21 and doxorubicin hydrochloride were co-loaded onto the PAMAM-modified HGNS (PAMAM-HGNS) by electrostatical adsorption. The loading of DOX was demonstrated by UV-visible absorption spectrum, energy dispersive spectrum analysis and confocal microscopy observation. And the complexation of as-miR-21 was proved by agarose gel electroph