苦参碱对表皮葡萄球菌密度感应系统ai-2信号分子的调控作用研究-regulation of matrine on ai - 2 signaling molecules in staphylococcus epidermidis density sensing system.docxVIP

Ab stractAntibiotics play an important role in treatment bacterial infection for a long time．But due to using antibiotics indiscriminately and irrationally，bacteria have serious drug resistance，even arisen multi drug resistant strains．Along with deeping research on the mechanism of bacterial drug resistance，it was found that drug resistance and bacterial biofilm have a great relationship．Once the biofilm is formed，bacterial resistance to antibiotics is increased over 1 000 times than that of planktonic bacteria．Bacterial quorum sensing system can regulate the bacterial biofilm formation，AI一2 signaling molecule play an importantrole in quorum sensing，thus quorum sensing signaling molecule was a new target for inhibitingbiofilm．This study chosen AI一2 signaling molecule as a research object，to study the formation rules of AI一2 signaling molecule and the effect characteristics of drug on AI-2 signaling molecule，then lay a foundation for the mechanism oftraditional Chinese medicine inhibit bacterial biofilm．First，we used different culture methods to culture planktonic and biofilm bacteria of Staph)7lococcus epidermidis；then used spectrophotometer and XTT method to test growth curve of planktonic bacteria and biofilm bacteria respectively．We detected transcription level of luxS gene in this two different growth pattern by real--time PCR,and measured AI·-2 signaling molecule activity byKharveyi bioluminescence assay．We use these methods to study the chang rule of AI-2 signalingmolecule activity and relative expression of luxS gene in different growth parttern．Mainly research how antibiotics，matrine and other traditional Chinese medicine regulate biofilm formation and AI一2 signaling molecule activity in two different cultivation mode of Staphylococcus epidermidis．Then study how glycerol monolaurate，n-nonanoic acid and combined use of matrine and this two compounds effect on biofilm formation and AI-2 signaling molecule．The result showed that AI-2 signaling molecule and th