六棱菊标准提取物和抗炎胶囊抗炎保肝作用分析-analysis of anti-inflammatory and hepatoprotective effects of liulingju standard extract and anti-inflammatory capsule.docxVIP

“抗炎胶囊”对角叉菜胶致大鼠足肿胀的影响···························……“抗炎胶囊”对小鼠毛细血管通透性的影响······························…………“抗炎胶囊”对小鼠棉球肉芽肿的影响·································……第三章“抗炎胶囊”体外保肝作用研究·······································原代肝细胞分离培养·······································……”……样品对原代肝细胞的毒性检测法··································……“抗炎胶囊”对致新生大鼠原代肝细胞损伤的影响············……一……“抗炎胶囊”对致新生大鼠原代肝细胞损伤的影响·········……“抗炎胶囊”对致新生大鼠原代肝细胞损伤的影响·········……“抗炎胶囊”对致新生大鼠原代肝细胞损伤的影响·········……“参考文献·················································································第三部分讨论········································································……小……·······················································································结……参考文献·················································································……附病理组织切片图·····································································第四部分综述········································································……植物源抗炎药物研究进展参考文献·················································································…………本人简历·················································································……博士期间发表论文·····································································……·················································································致谢浙江大学博士学位论文中文摘要，，。六棱菊作为一种民间药用植物具有清热解毒抗菌消炎等功效、，。、、疗效甚著该植物长期广泛应用于民间和中医实践中且资源丰富以“六棱菊”、。为主要成分的制剂在临床上治疗感冒扁桃体炎腮腺炎急性呼吸道感染等取，，得了显著疗效具有较高的研究和开发价值目前国内对六棱菊的药理和临床研，。究主要停留在其粗提物上缺乏药效物质基础相关研究而国外有关这种植物的。，研究报道很少总之国内外尚无这种植物抗炎保肝作用系统的研究报道本课，。题通过抗炎活性跟踪分离和标化这种植物的有效部位并对其标准提取物抗炎保”，肝作用进行系统研究以期为该植物的临床应用和深入开发打下基础，““抗炎胶囊”是一种民间植物药的有效部位抗炎胶囊的抗炎保肝作用研究。为本研究室与制药企业的合作开发项目本研究考察了“抗炎胶囊”的抗炎保肝，。作用为该胶囊的临床应用提供了部分依据第一部分六棱菊抗炎保肝作用研究、采用“二甲苯致小鼠耳肿胀模型”跟踪分离并比较六棱菊不同药用部位不，。同容剂及不同工艺提取物的抗炎活性结合急性毒性试验结果最终确定六棱菊的，。抗炎活性部位并对该部位进行标化急性毒性测定结果显示六棱菊提取物的。大于叭采用比色法测定六棱菊提取物的总酚含量为提取。，。物采用测定其主要成分为二咖啡酞奎尼酸二咖啡酞奎尼酸在提取物中，一。的含量为采用植化方法从提取物中分离鉴定了种二咖啡酞奎尼酸化合物，一二咖啡酞奎尼酸、，一二咖啡酞奎尼酸和二咖啡酞奎尼酸六棱菊提取物抗炎作用及其机理研究运用代表炎症发展个阶段的个动物模型和个免疫性炎症模型考察了六。、棱菊提取物的抗炎作用这些模型分别是代表炎症反应第一时相的二甲苯、致小鼠耳肿