卡培他滨对食管细胞系shee及sheec作用分析-analysis of capecitabines effect on she and sheac esophageal cell lines.docxVIP

下载本文档

5
0
约4.32万字
约 41页
2018-05-29 发布于上海
举报
版权申诉

卡培他滨对食管细胞系shee及sheec作用分析-analysis of capecitabines effect on she and sheac esophageal cell lines.docx

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

卡培他滨对食管细胞系shee及sheec作用分析-analysis of capecitabines effect on she and sheac esophageal cell lines

cells using with 10% blood serum contained drugs were 11.23% and 23.63% at 48h, it was significantly different (P0.05); The growth inhibiting ratios of SHEE and SHEEC cells using with 10.375ng/ml Fluorouracil, which concentration was same as Fluorouracil in the group of 10% blood serum contained drugs, were 10.57% and 9.98% at 48h, it was not significantly different (P0.05); the growth inhibiting ratios of SHEE and SHEEC cells using with 227.8ng/ml Doxifluridine, which concentration was same as Doxifluridine in the group of 10% blood serum contained drugs, were 1.69% and 16.76% at 48h, it was significantly different (P0.05); the growth inhibiting ratios of SHEE and SHEEC cells using with 10.375ng/ml Fluorouracil plus 227.8ng/ml Doxifluridine, which concentration was same as Fluorouracil plus Doxifluridine in the group of 10% blood serum contained drugs, were 1.69% and 16.76% at 48h, it was significantly different (P0.05).Conclusion: Cancerization of normal esophageal epithelium cell follows with increase of positive expression of TP. Capatibine has weak inhibition effect to SHEE cell line, selective lethal effect to SHEEC cell line. Expression of TP have no obvious correlation with chemo effect of 5-Fu.KEY WORDS: human esophageal cell line, capecitabine, thymidine phosphorylase, Fluorouracil, DoxifluridineDissertation Type: Basic type for applicationIV缩略语词汇表SHEEShantou human embryonic esophageal epithelial cell line人胚食管上皮细胞汕头株SHEECShantou human embryonic esophageal carcinoma cell line人胚食管上皮癌细胞汕头株TP5-Futhymidine phosphorylase Fluorouracil胸苷磷酸化酶氟尿嘧啶5-DFURDoxifluridine脱氧氟尿苷MTT M199Methylthiazoletrazolium Culture medium 199四甲基偶氮唑盐M199 培养基PBSPhosphate buffered saline磷酸盐缓冲液EDTAEthylene diaminetetraacetic acid乙二胺四乙酸DMSODimethyl sulfoxide二甲基亚砜ODOptical density光密度hhour小时33第1章综述胸苷磷酸化酶与恶性肿瘤关系的研究进展恶性肿瘤已成为对人类健康危害最大的疾病之一，其发生发展是多种因素共同作用的结果，如环境的恶化、遗传基因位点的突变以及机体免疫功能的下降等。恶性肿瘤新生血管的形成、生长在恶性肿瘤生长、浸润和转移过程中所起的作用已越来越受到重视。功能性血管为恶性肿瘤的生长提供氧和营养成分、保证其在局部生长的同时，也为肿瘤细胞的扩散提供途径，有研究资料显示，除非新生血管形