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Abstractplasmid.It can obtaine Nanobodies after using the IPTG inductionin in E. coli expression system. SDSelectrophoresis shows that the recombinant proteinmolecule weight is approximately 20 kDa in line with expectation.3 、 The method of using the quantum dots ibaoji recombinant antibodies forintracellular nano target detection is validated. It prepares of SP2/0 cells climbing film and uses the quantum fluorescent technology to identify Nanobodies and detects the expression of c-Myc protein in the cell.The cells shows fluorescence after adding the Nanobodies and quantum dots,but donot shows fluorescence only adding the quantum dots.This study of Nanobodiesresults can be expectly used to detect c-Myc protein expression in cells and then applied to diagnostic cancer cells.Keywords: Nanobody; c-Myc; ELISA; Quantum dots缩 略 语Abbreviations缩略语英文名中文名AmpAmpicillin氨苄青霉素BSABovine albumin牛血清白蛋白CAPChloramphenicol氯霉素DMFN,N-DimethylformamideN,N-二甲基甲酰胺DNADeoxyribonucleic acid脱氧核糖核酸 dNTPDeoxynucleoside triphosphate脱氧核苷三磷酸 ELISAEnzyme-linked immunosorbent assay酶联免疫吸附试验 Gly-HClGlycine-Hydrochloride甘氨酸-盐酸HRPHorseradish peroxidase辣根过氧化物酶IC50Half maximal inhibitory concentration of a substance半抑制浓度IPTGIsopropy-β-D-thiogalactoside异 丙 基 -β-D- 硫 代 半乳糖苷KanKanamycin卡那霉素KDaKilodalton千道尔顿LBLuria bertani mediumLB 培 养 基ODOptical density光密度值OVAOvalbumin鸡卵清白蛋白PBSPhosphate buffered saline磷酸盐缓冲液PCRPolymerase chain reaction聚合酶链式反应PEGPolyethylene glycol聚乙二醇QDsQuantumdots量子点QDs-SAQuantum dot labeled streptavidin-biotin量子点标记链霉亲和素scFvSingle chain antibody fragment单链抗体SDSSodium dodecyl sulfate十二烷基磺酸钠SDSSodium dodecyl sulfate polyacrylamide gel electrophoresis聚丙烯酰胺凝胶电 泳TrisThis(hydroxymethyl)aminomethane三羟甲基氨基甲烷VHHVariable domain of heavy chain of heavy chain antibody单域重链抗体目录摘 要 IAbstract II缩 略 语 IV第 1 章 前言 11.1 原癌基因 myc 概况11.1.1 原癌基因 11.1.2 原癌基因分类 11.1.3 myc 基因的结构与功能21.1.4 c-Myc 蛋白的作用机制41.1.5 c-myc 与肿瘤61.2 c-myc 在检测中的研究进展61.2.1 基于 c-myc 基因的检测方法61.2.2 基于 c-Myc 蛋白的检测方法71.2.3 c-Myc 作为蛋白融合标签的应用71.3 量子点