利福平致大鼠胆汁淤积适应过程的实验分析-experimental analysis of adaptation process of rifampin-induced cholestasis in rats.docxVIP

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利福平致大鼠胆汁淤积适应过程的实验分析-experimental analysis of adaptation process of rifampin-induced cholestasis in rats.docx

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利福平致大鼠胆汁淤积适应过程的实验分析-experimental analysis of adaptation process of rifampin-induced cholestasis in rats

（1）谷丙转氨酶（ALT）和谷草转氨酶（AST）测定20（2）总胆红素（TBIL）和直接胆红素（DBIL）测定20（3）碱性磷酸酶（AKP）测定212.6.3肝组织病理学检查·················································212.6.4肝组织超微结构观察··············································212.6.5肝组织匀浆制备····················································222.6.6肝组织总胆汁酸检测··············································222.6.7肝组织蛋白含量和肝匀浆SOD、MDA、GSH-PX测定··23（1）肝组织蛋白含量测定·············································23（2）肝匀浆SOD测定23（3）肝匀浆MDA含量测定（TBA法）24（4）肝匀浆GSH-PX242.7统计学方法····························································253.结果·········································································253.1利福平对大鼠一般情况的影响····································253.2大鼠血清生化指标的动态变化····································273.3大鼠肝脏大体形态及病理组织学变化···························303.4肝组织超微结构变化················································313.5大鼠肝组织总胆汁酸的变化·······································333.6大鼠肝组织MDA含量变化343.7大鼠肝组织SOD、GPX活性变化344.讨论·········································································354.1适应性现象的概念···················································354.2利福平致大鼠胆汁淤积模型的建立和特点····················374.3利福平致大鼠肝损伤的适应性现象·····························374.4利福平对大鼠肝组织病理学的影响·····························3824.5氧化应激在利福平致肝损伤过程中的作用·····················3834.6利福平致胆汁淤积适应性现象机制的进一步探讨···········404.7药物性肝损伤适应性现象的临床意义与困惑·················415.结论·········································································42参考文献······································································44附录············································································49个人简历······································································49致谢············································································50中英文缩略词表英文缩写英文全称中文名称DILIDruginducedliverinjury药物性肝损伤RIFrifampicin利福平CYPcytochromeP450细胞色素P450BSEPbilesaltexportpump胆汁酸输出泵ALPalkalinephosphatase碱性磷酸酶INRInternationalNormalizedRatio国际标准化比值ALTGlutamatePyruvateTransaminase谷丙转氨酶ASTGlutamic-oxal(o)aceticTransaminase谷草转氨酶TBILT