两种介孔硅分子印迹聚合物的制备及其应用分析-preparation and application analysis of two mesoporous silica molecularly imprinted polymers.docxVIP

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两种介孔硅分子印迹聚合物的制备及其应用分析-preparation and application analysis of two mesoporous silica molecularly imprinted polymers.docx

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两种介孔硅分子印迹聚合物的制备及其应用分析-preparation and application analysis of two mesoporous silica molecularly imprinted polymers

silica particles , as a solid phase extraction sorbent for acrolein biomarkers N-acetyl-S-(3- hydroxy-propyl)-L-cysteine (HPMA) pre-treatment ，and make the elution conditions ofthe molecular imprinted solid phase extraction(MIP-SPE) column conducted in-depth discussion.Through optimized on the sample 、washing and elution conditions of each step and compared with a blank column ： under the acidic conditions on the sample, with1mL5% formic acid/methanol solution washing, 1mL20% formic acid/methanol solution was effective eluted the target compound. Verify the accuracy and highly selective of the molecularly imprinted solid phase extraction method.The result showed that this molecularly imprinted polymer for 3-HPMA had highly selective , high affinity , it was suitable for solid-phase extraction separation and the MISPE operated simplely, separated efficient highly than other methods , and also reduced environmental pollution , it has a good application prospects.Keywords: Mesoporous Silica Polymer ; Preparation; Application目录摘要·········································································································· I Abstract ········································································································ II 目录·········································································································· I1.1 分子印迹技术·····················································································11.1.1 分子印迹技术概述······································································11.1.2 分子印迹基本原理······································································11.1.3 分子印迹聚合物的制备································································21.1.4 分子印迹聚合物的应用································································41.1.5 表面分子印迹技术······································································61.2 生物标记物及其研究进展····································································