lcarnosine对nit1胰岛细胞功能 增殖和凋亡影响的研究-study on the effect of lcrnosine on the functional proliferation and apoptosis of nit - 1 islet cells.docxVIP

culturedwithoutL-carnosine,whilenoglucosestimulation(P0.01).IncreasedproliferativeratewasdetectedinHgroupcomparedtoCgroup,thatis,anaugmentof63.59%±3.7%(P0.01),groupH+Aincreased23.44%±6.08%thangroupH(P0.01),groupH+Bincreasednotsignificantly(P0.05);apoptosisrate:Hgroupincreased75.15%±7.61%thanCgroup(P0.01),H+Agroupdecreased58.17%±6.92%thanHgroup(P0.01),H+Bgroupdecreasedsignificantly(P0.01),yethaveahigherapopticratethanH+Agroup.caspase-3mRNAlevelsinHgroupuppered(P0.05vsCgroup),inH+Agoupdeclined(P0.05vs.Hgroup),inH+Bgroupdelined(P0.05vs.Hgroup);whilebcl-2mRNAexpressioninHgroupdecreased(P0.05vsCgroup)andinH+Agroupincreased(P0.01vsHgroup),inH+Bgroupincreased(P0.05vs.Hgroup).Conclusion：(1)ChronichighglucoseconditioninhibitsinsulinsecretionofNIT-1cells,higherconcentrationsofL-carnosinecandirectlystimulateNIT-1cellsinsulinsecretion.Chronichighglucoseconditionincreasescellsproliferationandapoptosis,yettheoverallcellnumbersdeclined;lowernear-physiologicallevelsofL-carnosineforalongtimepromotescellsproliferationandreduceapoptosis,thereforehaveanincreasedcellnumbers;caspsase-3andbcl-2genesmaytakepartintheprocessofNIT-1cellsproliferationandapoptosis,andofpreservationofNIT-1cellsresultedfromL-carnosine.Keywords：L-carnosine；highglucose；proliferation；apoptosis；NIT-1cell中英文缩略词表Carnosinase1(CN1)二肽基肽酶carnosineL-肌肽PPARγ过氧化物酶体增殖活化受体γNODnonobesediabeticmice非肥胖型糖尿病鼠KRBBKrebs-RingerbicarbonatebufferKrebs-Ringer碳酸氢盐缓冲液BrdU5-溴脱氧尿嘧啶核苷RT-PCRreversetranscriptionpolymerasechainreaction逆转录聚合酶链反应mRNAmessengerribonucleicacid信使核糖核酸bpbasepair碱基对DEPCDiethylpyrocarbonate双碳酸二乙酯dNTPdeoxynucleosidetriphosphate脱氧核苷三磷酸ODopticaldensity吸光度RIAELISARadioimmunoassayenzyme-linkedimmunosorbentassay放射免疫法GSIS葡萄糖刺激的胰岛素释放AnnexinⅤ一种Ca+依赖的磷脂结合蛋白EBIGTT2DM澳化乙锭糖耐量受损2型糖尿病独创性声明本人声明所呈交的学位论文是我个人在导师指导下进行的研究工作及取得的研究成果。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体，均已在文中以明确方式标明。本人完全意识到本声明的法律结果由本人承担。学位论文作者签名：日期：年月日学位论文版权使用授权书本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有权保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅。本人授权华中科技大学可以将本学位论文的