UPLC法同时测定复方守宫散中蒽醌类含量-吴健,吴小明,高家荣,等.docVIP

UPLC法同时测定复方守宫散中蒽醌类含量-吴健,吴小明,高家荣,等 第16卷??第6期?????2014?年?6?月 JOURNAL OF LIAONING UNIVERSITY OF TCM 辽宁中医药大学学报 Vol. 16 No. 6 Jun .，2014 DOI：10.13194/j.issn.1673-842x.2014.06.022 UPLC法同时测定复方守宫散中蒽醌类含量 22 吴健1，，吴小明1，高家荣1，，韩燕全1，罗欢1 （1.安徽中医药大学第一附属医院，国家中医药管理局中药制剂三级实验室，安徽?合肥?230031； 2.安徽中医药大学，安徽?合肥?230032） 摘 要：目的：建立UPLC法同时测定复方守宫散中大黄素、大黄素甲醚含量的方法。方法：用Acquity?BEH? C18（2.1?mm×100?mm，1.7?μm），甲醇-0.1%磷酸水溶液（80∶20）为流动相，流速：0.25?mL/min，柱温：26?℃，检测波长：254?nm。结果：大黄素、大黄素甲醚的进样量分别在0.0398~0.6625?μg（r=0.9996）、0.0480~0.8000?μg（r=0.9994）范围内与其峰面积呈良好的线性关系；平均回收率分别为97.07%、98.24%，RSD分别为2.26%、3.27%。结论：该方法快速、可靠、准确，可作为复方守宫散的质量控制方法。 关键词：超高效液相色谱；复方守宫散；大黄素；大黄素甲醚；含量测定 (2014) 06- 中图分类号：R284 文献标志码：A 文章编号：1673-842X 0060- 03Simultaneous Determination of Anthraquinone Contents in Compound Shougong Powder by UPLC 22 WU Jian1，，WU Xiaoming1，GAO Jiarong1，，HAN Yanquan1，LUO Huan1 （1.The First Affiliated Hospital of Anhui University of Chinese Medicine，Grade 3 Laboratory of Traditional Chinese Medicine Preparation，State Administration of Traditional Chinese Medicine，Hefei 230031，Anhui，China；2.Anhui University of Chinese Medicine，Hefei 230032，Anhui，China） Abstract：Objective：To establish a UPLC method for the determination of Emodin and Physcion in Compound Shougong Powder. Methods：The determination was performed on Acquity BEH C18（2.1 mm×100 mm，1.7 μm）with mobile phase consisted of Methabol-0.1% phosphoric acid aqueous solution（80：20）at a flow rate of 0.25 mL/min. Column temperature was 26℃. The UV detection wavelength was set at 254 nm. Result：The calibration curves were linear in the range of 0.0398~0.6625 μg（r=0.9996）for Emodin，0.0480~0.8000 μg（r=0.9994）for Physcion. The average recovery was 97.07%，98.24% and RSD was 2.26%，3.27% respectively. Conclusion：The determination method is fast，reliable，accurate，and it can be used for quality control of Compound Shougong Powder. Key words：UPLC；Compound Shougong Powder；Emodin；Physcion；cont