口腔鳞状细胞癌基质金属蛋白酶表达及其与血管生成的关系_论文.docxVIP

口腔鳞状细胞癌基质金属蛋白酶9表达及其与血管生成的关系 【摘要】 　　［目的］探讨口腔鳞状细胞癌（OSCC）发生、发展过程中基质金属蛋白酶9（MMP9）对微血管生成的意义。［方法］采用免疫组化SP法对65例OSCC、40例非典型增生、22例正常口腔黏膜进行MMP9、CD34免疫组化染色。检测OSCC、非典型增生、正常口腔黏膜中MMP9的表达和微血管密度（MVD），并分析MMP9的表达与MVD之间，以及它们与OSCC临床病理特征之间的关系。［结果］OSCC组中MMP9的表达与MVD值均高于非典型增生组，在非典型增生组中均高于正常口腔黏膜组；OSCC组、非典型增生组和正常口腔黏膜组中MMP9表达与MVD有正相关趋势；MMP9的表达与肿瘤大小、淋巴结转移密切相关，MVD与淋巴结转移密切相关。［结论］MMP9可能是OSCC的重要促血管生成因子之一。 【关键词】 口腔鳞状细胞癌　免疫组织化学　基质金属蛋白酶9　CD34 Correlation between Expression of Matrix Metalloproteinase9 and Angiogenesis in Oral Squamous Cell Carcinoma Abstract: ［Purpose］ To study the significance of matrix metalloproteinases9 (MMP9) on micro angiogenesisin carcinogenesis and progression of oral squamous cell carcinoma (OSCC). ［Methods］MMP9 and CD34 were detected by immunohistochemistry SP method in 22 cases of normal oral mucosa, 40 cases of atypical hyperlasia and 65 cases with OSCC. The expression of MMP9 and microvessel density (MVD) were detected in the three groups and the relationship between expression of MMP9 and MVD and its relationship with OSCC clinicpathological characteristics were reviewed.［Results］ The expression of MMP9 and the level of MVD in OSCC were significantly higher than that in atypical hyperlasia. The expression of MMP9 and the level of MVD in the group of atypical hyperlasia were significantly higher than that in normal oral mucosa. The expression of MMP9 were closely related with MVD in the tumor group, atypical hyperlasia group and normal oral mucosa group. MMP9 expression in OSCC was significantly associated with lymph node metastasis and tumor size. MVD in OSCC was significantly associated with lymph node metastasis. ［Conclusion］ MMP9 may be one of important factors in angiogenesis ofOSCC. Key words: oral squamous cell carcinoma; immunohistochemistry; matrix metalloproteinases9; CD34 肿瘤内新生血管的生成是影响肿瘤增殖和转移的重要因素，近年来的研究表明[1]基质金属蛋白酶（matrix metalloproteinases， MMPs）在肿瘤血管生成中有重要作用，明胶酶是MMPs的一个亚类，包括基质金属蛋白酶2（MMP2）和基质金属蛋白酶9（MMP9），其中MMP9与肿瘤血管生成的关系研究较少，研究结果也不