二氢青蒿素抑制fe2依赖的卵巢癌细胞生长及其联合cddp抗肿瘤作用的实验研究-experimental study on dihydroartemisinin inhibiting growth of fe2 - dependent ovarian cancer cells and its antitumor effect in combination with cddp.docxVIP

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二氢青蒿素抑制fe2依赖的卵巢癌细胞生长及其联合cddp抗肿瘤作用的实验研究-experimental study on dihydroartemisinin inhibiting growth of fe2 - dependent ovarian cancer cells and its antitumor effect in combination with cddp.docx

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二氢青蒿素抑制fe2依赖的卵巢癌细胞生长及其联合cddp抗肿瘤作用的实验研究-experimental study on dihydroartemisinin inhibiting growth of fe2 - dependent ovarian cancer cells and its antitumor effect in combination with cddp

Abstractobjective: This study was to explore the inhibition of DHA on Fe2 + -dependent growth of A2780 cisplatin sensitive strains and drug resistance strains of ovarian cancer cells and their anti-cancer effect in combination with CDDP as well as the related mechanism.results:1、Half inhibitory concentration (IC50) of DHA to A2780 and A2780CIS in72 h were 46.34±3.47μmol/L, 97.00±3.82μmol/L. 10% inhibitory concentration (IC10) were 1.1±0.16μmol/L, 1.8±0.22μmol/L. The effect of CDDP is 5.34 times to DHA of A2780 cells and 4.37 times of A2780CIS cells.2、IC50 of CDDP combinattion with IC10 of DHA to A2780 and A2780CIS cell lines were 11.23±1.40 μmol/L, 26.85±2.02μmol/L, the effection of DHA for the A2780 and A2780CIS was not very different from CDDP .3、The inhibition rate of growth of CDDP combinattion with DHA in their own IC20, IC30, IC40,IC50 concentration to A2780 and A2780CIS cells are low than use CDDP only, this action of cell survival is concentration-dependence, A2780 and A2780CIS has no significant difference.4、After dealing with deferoxamine in 0, 25, 100, 200 umol/L concentration for 6 h, survival rate of DHA on A2780 and A2780CIS cell rise, high concentration (100, 200 umol/L), treatment group increased more significantly.5、The clone formation rate of A2780 and A2780CIS under the effect of CDDP incombination with DHA are lower than single drug group of CDDP, with higher concentration, clone formation rate of the two cell lines were lower, this trend in DHA group is most obvious，in high concentration (IC40 / IC50) it almost declined close to the level of CDDP.6、The apoptosis rate of A2780 and A2780CIS uses CDDP in combination with DHAare higher than CDDP single drug group (P 0.05), according to resoult of DHA + CDDP on A2780 and A2780CIS group, there is also a difference of apoptosis rate between two time group of A2780 and A2780CIS , both for higher apoptosis rate of 24 h than 6 h .conclusions:1、Dihydroartemisinin has a killing effect for ovarian cancer A2