菲律宾蛤仔蒸煮液多糖的提取 分离及其性质的分析-extraction and separation of polysaccharide from clam cooking liquor and analysis of its properties.docxVIP

AbstractChina is one of the biggest countries of shellfish farming, processing and consuming. The yield of shellfish makes up 80% of total mariculture production, it is an important part of the marine aquaculture industry. Clam cooking liquid is the by-products of clam processing. A lot of the cooking liquid is directly poured into sea, which would result in the waste of resources and pollution. In the paper, clam cooking liquid as raw material was concentrated by the different temperature rotary evaporation concentrated manner, the polysaccharide in which extracted using alcohol precipitation after acid precipitation and direct alcohol precipitation method. Then the products were purified by DEAE-Sepharose Fast Flow anion exchange chromatography, getting two components, the compositions of monosaccharide were measured by high-performance liquid chromatography.Conclusions as follows:The content of soluble solids in the clam cooking liquid was 1.6%, and the contents of protein and polysaccharide were 0.57% and 0.74% respectively. The polysaccharide accounted for 46.3% of soluble solids.Clam cooking liquid was hydrolyzed by papain and Alcalase. When the pH and reaction temperature were certain, with the amount of enzyme (2.5%, 5%, 10%, 20%) increased and hydrolysis time (1h, 2h, 4h, 6h) extension, polysaccharide contents of digested by papain enzyme hydrolysis were similar with the undigested, indicating that the enzyme had no obvious effect on increasing the content of polysaccharide in clam cooking liquid.In different temperature concentrated manner, the cooking liquid using alcohol precipitation after acid precipitation could significantly increase the amount of extraction of crude polysaccharide, compared with the original cooking liquid extraction amount, containing a respective increase of 11.1% and 14.1%; the amount of extraction of crude polysaccharide increased 10.2% and 5.4% by direct alcohol precipitation method. The extraction method of crude polysaccharid