肺癌射频消融后cd4+t细胞亚群变化及其临床意义的初步探讨-preliminary study on changes of cd4 + t lymphocyte subsets after radiofrequency ablation of lung cancer and its clinical significance.docxVIP

下载本文档

8
0
约4.82万字
约 56页
2018-05-29 发布于上海
举报
版权申诉

肺癌射频消融后cd4+t细胞亚群变化及其临床意义的初步探讨-preliminary study on changes of cd4 + t lymphocyte subsets after radiofrequency ablation of lung cancer and its clinical significance.docx

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

肺癌射频消融后cd4t细胞亚群变化及其临床意义的初步探讨-preliminarystudyonchangesofcd4tlymphocytesubsetsafterradiofrequencyablationoflungcanceranditsclinicalsignificance

AbstactPurposePreliminarystudyonperipheralfourdistinctCD4+T-cellsubsetsincludingTh1,Th2,Th17andTreginhealthyindividual.AndpreliminarystudyontheclinicalsignificanceanddynamicchangeofCD4+T-cellsubsetsinlungcancerpatientsbeforeandaftertheRadiofrequencyablation(RFA)therapy.MaterialsandMethodTheperipheralheparinizedbloodsampleswereobtainedfrom30healthycontrolsand6patientswithlungcancerbeforeand0.5,1,2,3monthafterRFA.PercutaneousRFAwereperformedundercomputedtomographic(CT)-guidedandintravenousanesthesia.FourdistinctCD4+T-cellsubsetsTh1,Th2,Th17,Tregwereassessedbyflowcytometry.IFN-γ,IL-4,IL-17wereusedasmarkersforthedifferentiationofTh1,Th2,Th17subsets,respectively,usingthewholebloodintracellularstainingassaytoanalyzethecytokines.ThesebloodsampleswereaddedPMA,Ionomycin,Brefeldin,thenincubated6hours.Thesethreesubsetswerelabeledfluorochrome-coupledantibodysCD3—PE-Cy5,CD8—APC-Cy7,IFN-γ—APC,IL-17—FITC,IL-4—PE,anddetectedbyBDFACSCanto,analyzedbyFACSDivasoftware.ThecharacteristicofTregsubsetwasCD3+CD4+CD25+foxp3+,labeledfluorochrome-coupledantibodysCD3—APC,CD4—PE-Cy5,CD25—FITC,foxp3—PE,detectedbyBDFACSCanto,andanalyzedbyFACSDivasoftware.Dataofhealthycontrolswerepresentedasmeans±SD,analysisedbyStatisticalsoftwareSPSS13.0.ResultThelevelsofTh1,Th2,Th17andTregsubsetsinperipheralbloodofhealthyindividualswere:36.88%±9.43,1.01%±0.56,3.03%±1.00,1.83%±1.07,respectively,andTh1/Th2ratioswas53.09±38.35.BeforeRFAtherapy,thelevelofTh1cellandTh1/Th2ratioofpatientswerelowerthanhealthycontrol,whileTh2,Th17,Tregcellsubsetswerehigher.HalfmonthafterRFAtherapy,ThelevelofThlcellincreased,whileTh2,Th17andTregcellsdecreasedcomparedwithpreoperativelevels.Afterthen,Thetendencyfluctuate.However,thelevelofThlcellswasstillhigherthanpreoperativelevels,andTh2,Th17andTregwerestilllower.Th1/Th2ratioalsoincreased0.5monthafterRFAtherapy.ConclusionTheperipherallevelofTh1cellinhealthyindividualwashigherthanTh2,Th17,Treg.Inpatientswithlungcancer,thelevelsofTh1decreaseandTh2increase,leadingtotheimbalancestateofThl/Th2