肺痨康治疗耐药性肺结核的药效学分析--体外抑菌实验及对症实验-pharmacodynamics analysis of antituberculosis kang in treating drug-resistant pulmonary tuberculosis - in vitro bacteriostasis experiment and symptomatic experiment.docxVIP

目的：运用现代科学研究技术观察中药复方肺痨康的体外抑菌作用和部分对症治疗效果。方法：参照《化学治疗实验法》中的“抗结核药物实验法”在体外抑菌实验中测定肺痨康的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。采用细菌内毒素致家兔发热法观察肺痨康的解热作用；用汗液着色法、汗腺上皮组织形态观察法观察其止汗作用；用小鼠断尾法、毛细玻璃管法观察其止血作用。结果：①肺痨康高、中浓度时对H37RV、耐INH、耐RFP、耐RFP+INH菌株的作用明显，特别是对耐RFP菌株有较好的抑菌、杀菌效果；但对耐SM菌株的抑菌效果差。②肺痨康高、中剂量组可降低内毒素所致发热家兔的体温，且与阳性组无显著性差异（P＞0.05）。③肺痨康高剂量组能降低正常大鼠足趾汗腺上皮组织空泡发生率（P＜0.01），肺痨康高剂量组与阳性组比较有显著性差异（P＜0.01）。④肺痨康高、中剂量组均能降低小鼠出凝血时间，且肺痨康高剂量组与阳性组比较无显著性差异（P＞0.05）。结论：肺痨康在体外对常见耐药菌株有较好的抑菌效果；具有解热、止汗及止血功效。关键词：肺痨康耐药性肺结核药效学抑菌解热止汗止血Objective:Basedonscientifictechniques,observingbacteriostaticactioninvitroandpartialsymptomatictreatmenteffectofChineseCompoundFeilaokang.Method:ReferringtoMICandMBCofantituberculosisdrugsexperimentationinChemotherapyExperimentation;observingtheantipyreticeffectbasedoninducingrabbitsfeverwithbacterialendotoxin;observingrestraintsweatwithsweatcoloringmethodandsweatglandepithelialtissuemorphologyobservationmethod;observinghemostaticactionwithmiceseveredtailmethodandglasscapillarymethod.Results:①theH37RV、INH-resistantandRFP-resistantstrainareinfluencedobviouslywhenFeilaokangishigh-concentrationandmiddleconcentration,especiallyforRFP-resistantstrain.②Feilaokangcoulddecreasetemperaturethatwasinducdbytoxinofrabbitswhenitishighdoseandmiddledose,therewasnosignificantdifferencecomparedtopositivegroup（P＞0.05）.③Feilaokangcoulddepresstheincidenceofnormalrat’stoesweatglandepithelialtissuecavitationwhenitisanydose（P＜0.01）,therewassignificantdifferenceasFeilaokanghighdosegroupcomparedtopositivegroup（P＜0.01）.④Feilaokangcoulddecreasehemorrhageandcoagulationtimewhenitishighdoseandmiddledose,therewasnosignificantdifferenceasFeilaokanghighdosegroupcomparedtopositivegroup（P＞0.05）.Conclusion:Feilaokangputbetterantibacterialeffectoncommondrugresistantstrain;Feilaokanghaseffectofantipyretic、hemostasis、detersweat.Keywords:Feilaokang；drug-resistantpulmonarytuberculosis；pharmacodynamics；bacteriostasis；relievefever；detersweat；hemostasisefficacy英文缩略词表缩略词英文全称中文全称cAMPINHMBCMICcyclicadenofinemonophosphateIsonicotinylhydrazideM