抑郁症CUMS模型大鼠大脑白质毛细血管的体视学.doc

抑郁症模型大鼠大脑白质毛细血管的体视学研究 陈林木12，王飞飞12，谭川雪12，高原3，黄春霞4，张毅12，蒋林12，周春妮12，张蕾12，晁凤蕾12，唐勇12* （重庆医科大学 1.组织学与胚胎学教研室； 2.干细胞与组织工程学研究室； 3. 重庆医科大学附属第一医院老年科； 4.生理学教研室,重庆400016） 摘要：目的 探讨抑郁症大鼠大脑白质及白质内毛细血管的改变。方法 选取雄性SD大鼠，经筛选后随机分为对照组和模型组各10只。模型组给予5周的慢性不可预知性刺激，空白组不给予处理。造模成功后，分别从模型组和对照组中各随机选择5只SD大鼠运用免疫组化方法和体视学方法对大脑白质及其内毛细血管进行定量研究。结果 与对照组相比，模型组的大脑白质内毛细血管总体积和总长度分别下降了22.1%和29.9%，具有统计学差异（P0.05）。白质体积和毛细血管总表面积分别下降了8.1%和16.6%，不具有统计学差异（P＞0.05）。结论 抑郁症模型大鼠大脑白质毛细血管存在改变，这一结果为抑郁症血管因素的机制研究提供了重要的参数依据，也为临床抗抑郁药物的开发提供了新的研究方向。 关键字：抑郁症；白质；毛细血管；免疫组织化学；体视学 中图法分类号： R322 〔文献标识码〕 A ( Stereological Study of Capillary Changes in the White Matter of Depression Model of Rat Chen Linmu1,2,Wang Feifei1,2,Tan Chuanxue1,2,Gao Yuan3,Huang Chunxia4,Zhang Yi1,2,Jiang Lin1,2,Zhou Chunni1,2,Zhang Lei1,2, Chao Fenglei1,2，Tang Yong1,2* (1.Department of Histology and Embryology; 2.Laboratory of Stem Cell and Tissue Engineering; 3.Departmeng of Geriatrics, the First Affiliated Hospital; 4.Department of Physiology, Chongqing Medical University，Chongqing 400016, China) [Abstract] Objective To explore the white matter changes and the change of the capillaries in the white matter of depression model of rats. Methods After being screened, male SD rats were randomly divided into the control group and the model group with 10 rats each group. The depression model of rats was made by giving rats chronic unpredictable mild stimulation for five weeks. After the depression model of rats was successfully made, 5 rats were randomly selected from each group. The total volumes of the white matter and the total length, total volume, and total surface area of the capillaries in the white matter were investigated with the stereological techniques and immunohistochemistrical technique. Results Compared with the control rats, the total volume and total length of the capillaries in the white matter of the depression rats were significantly decreased by 22.1% and 29.9%, respectively（P0.05）. However there were?no sign