senp1对胆管癌细胞增殖与迁移能力的影响以及senp1在胆管癌标本中的表达情况-effect of sen p1 on proliferation and migration of cholangiocarcinoma cells and expression of sen p1 in cholangiocarcinoma specimens.docxVIP

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senp1对胆管癌细胞增殖与迁移能力的影响以及senp1在胆管癌标本中的表达情况-effect of sen p1 on proliferation and migration of cholangiocarcinoma cells and expression of sen p1 in cholangiocarcinoma specimens.docx

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senp1对胆管癌细胞增殖与迁移能力的影响以及senp1在胆管癌标本中的表达情况-effect of sen p1 on proliferation and migration of cholangiocarcinoma cells and expression of sen p1 in cholangiocarcinoma specimens

目录目录················································································1-2中文摘要············································································3英文摘要············································································4第一章前言······································································5-7第二章实验材料······························································8-12细胞株···········································································8主要仪器及设备·····························································8-9主要试剂····································································9-11主要溶液的配制··························································11-12质粒与菌种····································································12第三章实验方法·····························································13-21感受态细胞的制备与转化··················································13细胞培养···································································13-14质粒扩增···································································14-16质粒转染·······································································16质粒的提取································································16-17WesternBlot检测基因蛋白水平表达17-18细胞总蛋白质的提取························································17SDS电泳17WesternBlot分析17-18RNA提取18-19RNA逆转录实验19荧光定量PCR实验（Real-TimePCR实验）19细胞划痕实验（Woundedhealing)19细胞迁移实验(transwell)19-20细胞毒性实验（MTT法）20统计学方法·································································20-21第四章实验结果·····························································22-311.过表达SENP1后胆管癌细胞株增殖与迁移能力增强·······22-252.敲低SENP1水平后胆管癌细胞增殖和迁移能力降低25-273.过表达与敲低SENP1后检测AIB1的变化27-314.提取胆管癌手术标本总蛋白检测SENP1含量31第五章讨论···································································32-34第六章结论·······································································35参考文献·······································································36-38综述··························································