野生型 vhl 基因提高肾细胞癌细胞786 o 对 trail 敏感性的实验研究-experimental study on wild type vhl gene increasing sensitivity of renal cell carcinoma 786 o to trail.docxVIP

独创性声明本人郑重声明，本学位论文是本人在导师指导下进行的研究工作及取得的研究成果的总结。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体，均已在文中以明确方式标明。本人完全意识到本人将承担本声明引起的一切法律后果。学位论文作者签名：日期：年月日学位论文版权使用授权书本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有权保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅。本人授权华中科技大学可以将本学位论文的全部或部分内容编入有关数据库进行检索，可以采用影印、缩印或扫描等复制手段保存和汇编本学位论文。本论文属于保密□，在年解密后适用本授权书。不保密□。（请在以上方框内打“√”）学位论文作者签名：指导教师签名：日期：年月日日期：年月日目录摘要························································································1第一部分摘要·······················································································1第二部分摘要·······················································································3Abstract4正文第一部分野生型VHL基因提高肾细胞癌细胞786-O对TRAIL敏感性的基础研究序言································································································7材料与方法····························································································9实验结果·····························································································26讨论································································································33结论和展望··························································································46参考文献·····························································································48第二部分综述···································································································59参考文献·····························································································64附录1攻读博士学位期间发表的论文目录······································70附录2主要英文缩略词表·······························································72致谢······························································································74中文摘要第一部分实验研究野生型VHL基因提高肾细胞癌细胞株786-O对TRAIL敏感性的基础研究目的：91%的散发性肾细胞癌患者中存在肿瘤抑制基因vonHippel-Lindau(VHL)的突变或失活。VHL基因以及翻译的产物pVHL能够在转录和翻译水平调节多种与肾细胞癌发生发展相关基因的表达，但与TRAIL介导的肿瘤细胞凋亡之间的关系并不明确，本次研究的目标是在肾细胞癌786-O细胞株上明确野生型VHL基因在TRAIL介导的肿瘤细胞凋亡中的作用。方法：我们选用肾细胞癌786-O细胞株、单克隆携带野生型VHL基因全长