银杏外种皮中银杏酸抗植物病原真菌活性研究.pdfVIP

( ) ( ) ( ) 江苏大学学报 医学版 　Academic Journal of Jiangsu University medicine ,October 2003 ,13 5 3·89 · 银杏外种皮中银杏酸抗植物病原真菌活性研究 1 2 3 2 2 吴向阳 , 仰榴青 , 陈　钧 , 付海珍 ,夏国华 (江苏大学 1. 化学化工学院;2. 药学院;3. 生物与环境工程学院 ,江苏 镇江 212013) [摘 　要] 　目的:研究银杏酸对植物病原真菌的抗菌活性 ,为银杏外种皮的开发利用提供基础 。方法 :用石油醚回流提取银 杏外种皮得粗提物 ,再经硅胶柱色谱分离 、纯化得银杏酸混合物 ,用 HPLC 法分析了其中银杏酸的含量 ,采用菌落生长直径测 定法测定了它们对 12 种病原真菌的抗菌活性 。结果 :粗提物和银杏酸混合物中银杏酸含量分别为 58. 6 %和 86. 3 % ,它们对 多种病原真菌都有活性 ,具有相同的抗菌谱 ,两样品对花生网斑病菌生长的抑制率最高 。结论 :银杏外种皮中银杏酸是抗菌 活性成分之一 ,还含有其他抗菌活性物质 ,可开发成生物农药 。 [ 关键词] 　银杏外种皮 ; 银杏酸 ; 病原真菌 ; 抗菌活性 ( ) [ 中图分类号] 　R284. 2 　　[文献标识码] 　A 　　[文章编号] 　1671 - 7783 2003 05 - 0389 - 03 Study on the Antifungal Activity of Ginkgolic Acids on Patho genic Fungi in Ginkgo Biloba Sarcote sta 1 2 3 2 2 W U Xian gyan g , YA N G L iuqing , CHEN J un , FU Haiz hen , X IA Guohua ( 1. School of Chemist ry and Chemical Engineering ; 2. School of Pharm ay ; 3. School of Biology and Environmental Engineering , Jiangsu U niversity , Zhenjian g Jiangsu 212013) [ Abstract ] 　Objective : For providing with basis in developing Ginkgo biloba sarcotesta , antifungal activity of ginkgolic acids ( GA) on patho genic fungi was studied. Methods : The crude extract was obtained by light petro leum reflux extraction. The mixture of GA could be separated by silica gel column. GA was determined by HPLC. The antifugal activities of two samples on twelve kinds of patho genic fungi were studied. Results : The GA contents of the crude extract and the GA mixture was 58. 6 % and 86. 3 % respectively. They have antifun gal activities on most of fung