玉米赤霉烯酮对断奶仔猪卵巢中ghr ghrelin和pcna 表达的影响-effect of zearalenone on ghrelin and pcna expression in ovaries of weaned piglets.docxVIP
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玉米赤霉烯酮对断奶仔猪卵巢中ghr ghrelin和pcna 表达的影响-effect of zearalenone on ghrelin and pcna expression in ovaries of weaned piglets
关于学位论文原创性和使用授权的声明本人所呈交的学位论文,是在导师指导下,独立进行科学研究所取得 的成果。对在论文研究期间给予指导、帮助和做出重要贡献的个人或集体, 均在文中明确说明。本声明的法律责任由本人承担。本人完全了解山东农业大学有关保留和使用学位论文的规定,同意学 校保留和按要求向国家有关部门或机构送交论文纸质本和电子版,允许论 文被查阅和借阅。本人授权山东农业大学可以将本学位论文的全部或部分 内容编入有关数据库进行检索,可以采用影印、缩印或其他复制手段保存 论文和汇编本学位论文,同时授权中国科学技术信息研究所将本学位论文 收录到《中国学位论文全文数据库》,并向社会公众提供信息服务。保密论文在解密后应遵守此规定。论文作者签名: 导师 签 名: 日期: 符 号 说 明英文简称英文全称中文全称AFTaflatoxin黄曲霉毒素AGEagarose gel electrophoresis琼脂糖凝胶电泳AGFatretic growing follicle闭锁的生长卵泡AOACassociation of analytical communities美国官方分析化学师协会APrFatretic primordial follicle闭锁的原始卵泡bpbase-pairs碱基对CPcrude Protein粗蛋白DEPCdiethypyrocarbonate焦炭酸二乙酯DONdeoxynivalenol呕吐毒素EBethidium bromide溴化乙锭ERestrogen receptor雌激素受体FSHfollicle-stimulating hormone卵泡刺激素FUMfumonisin烟曲霉毒素GAPDHglyceraldehyde-3-phosphate dehydrogenase甘油醛-3-磷酸脱氢酶GCgranulosa cell颗粒细胞GEgross energy总能量GHgrowth hormone生长激素GHRgrowth hormone receptor生长激素受体GHSR1agrowth hormone secretagogue receptor type 1a生长激素促分泌素受体 1a 型HEhematoxylin-esoin staining苏木精-伊红染色法HGFhealthy growing follicle健康生长卵泡HPLChigh performance liquid chromatography高效液相色谱法HPrFhealthy primordial follicle健康原始卵泡HRPhorseradish eroxidase辣根过氧化物酶 IHCimmunology and histology chemistry免疫组织化学 IODintegrated optical density累积光密度kDakilobit Dalton千道尔顿LHluteinizing hormone黄体生成素NRCnational Research Council(美)国家科学委员会PBSphosphate buffer saline磷酸盐缓冲液 PCNAproliferating cell nuclear antigen增殖细胞核抗原 PCRpolymerase chain reaction聚合酶链式反应ZENzearalenone玉米赤霉烯酮ZOLzearalenol玉米赤霉烯醇α-ZOLα-zearalenolα-玉米赤霉烯醇β-ZOLβ-zearalenolβ-玉米赤霉烯醇目录中 文 摘 要...............................................................................................................................IABSTRACT ........................................................................................................................... III1 前 言...................................................................................................................................... 11.1 ZEN 的研究现状11.1.1 ZEN 生物学特性及污染现状11.1.2 ZEN 在体内的代谢、转化和残留21.1.3 ZEN 的生殖毒性3GHR6GHR 的结构61.2.2 GHR 在卵巢中的分布与作用61.
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