玉米茎基病菌生物学特性及品种抗病性的分析-analysis of biological characteristics and disease resistance of maize stem-based pathogen.docxVIP

AbstractThe corn basal stem rot is a main disease of maize, In the spring maize area of northeast,north, northwest of China, Huang-Huai-Hai summer maize region and southwestern hills and mountains where maize growing,the occurrence is widespread,and seriously affect the quality and yield of maize[1]. The maize stem base rot is a soil, seed-borne disease that infection by alone or mixed Pythium and Fusarium. In order to grasp the culture and propagation technique of corn stem base rot bacteria in Anhui province, provide better technical service for Resistance Identification,this article studied the biological characteristics of corn basal stem rot in Anhui Province and had carried disease identification of validation on maize. And also provide a scientific basis for the disease resistance maize breeding,and the rational distribution of disease-resistant varieties in Anhui province.The F.graminearum Schawbe is from two different places and the (P.aphanidermatum(Eds.)Fitzp is from three different places. This paper studied its biological characteristics and the virulence influence of different bacteria inoculation on the maize varieties; and carried on the resistant identification of 19 Introduction varieties and 74 for the test for examination of varieties of Anhui Province, selected suitable resistant varieties for cultivation in Anhui Province.The main results are as follows:1、The optimization of corn basal stem rot medium formulationBy measuring the different culture methods have an effect on corn basal stem rot ((P.aphanidermatum(Eds.)Fitzp) mycelial growth rate. The results showed that no significant difference in the mycelial growth rate of the strain in the two basal medium of PDA and PCA; when the C source in the basal medium is sucrose , mycelial growth rate is highest, followed by starch, maltose and glucose; the N source in the medium is Ca (NO3) 2, mycelial growth rate highest, followed by NH4NO3, NH4Cl; carbon content of sucrose in the basal medium is