预输注5-fu处理的同种异基因淋巴细胞诱导大鼠肝移植免疫耐受的初步研究-preliminary study on immune tolerance induced by allogeneic lymphocytes pretreated with 5 - fu in rat liver transplantation.docxVIP

广州医学院硕士研究生学位论文 广州医学院硕士研究生学位论文 PAGE PAGE 6 others were included in the survival analysis. 5. The expression of FasL,CD4 and CD8 in the grafts were detected by immunohistochemical staining method. The Imag-pro Plus 6.0 software was used to analyze the images． Results Survival time of group C was 19.0±5.3d, longest than the other groups(P0.05). There was no difference between group B(10.3±2.7d) and group D(11.7±3.9d), and survival time of group A(6.7±1.5d) was shortest(P0.05). Pathologies: Mild acute rejection response was observated in the liver of group C. hepatic lobule existed and hepatocyte arranged neatly. central vein and header was invased by some inflammatory cells. group A showed serious acute rejection response, group B and D showed moderate rejection response. athological changes in group C was slighter than other groups. The expression of CD4 and CD8： In acini hepatis, the IOD SUM of CD8 in group A was higher than other groups(P0.05). There were no significant difference in group C,group B and group D(P0.05). In the header,the IOD SUM of CD8 in group A was higher than other groups(P0.05), and the lowest in the group C. There were no significant difference between group B and group D. The expression of CD4 in the graft was negative. The expression of FasL：In the acini hepatis and header, the IOD SUM of FasL in group C was higher than other groups(P0.05), and the lowest in the group A. There were no significant difference between group B and group D. Conclusion The model of orthotopic liver transplantation was established from Wistar to SD rats. In the acute rejection of rat liver allograft, CD8+T lymphocytes played a key role, while CD4+T lymphocytes was adjunctive. Pre-infusion of lymphocytes treated by low level 5-Fu can enhance the expression of FasL on recipients liver cell after liver transplantation. The mechanism, that immune tolerance induction in rat liver transplantation by pre-infusion of allogeneic lymphocytes treated by 5-Fu, may be ： ind