COX-2和Bax在非小细胞肺癌中表达及意义.docVIP

COX-2和Bax在非小细胞肺癌中表达及意义 　　【摘要】 目的 探讨非小细胞肺癌(NSCLC)中环氧化酶-2(COX-2)和Bax的表达及其临床意义。方法 采用免疫组织化学方法检测57例NSCLC癌组织（腺癌/鳞癌）、相应配对的15例正常组织及57例淋巴结COX-2蛋白和Bax蛋白的表达水平，计算其阳性百分比进行相关性分析。结果 NSCLC组织中COX-2蛋白表达高于癌旁正常组织(P0.05)；Bax蛋白表达低于癌旁正常组织(P0.05)。结论 COX-2在NSCLC尤其是肺腺癌中表达上调,Bax蛋白在NSCLC组织中表达过低，COX-2可能通过降低Bax表达，抑制细胞凋亡参与NSCLC的发生、发展。? 　　【关键词】 非小细胞肺癌；环氧化酶-2；Bax?? 　　Expression and significance of cyclooxygenase-2 and apoptosis inhibitor-Bax in Non-small cell lung cancer TONG Xu, SONG Zhuo.The Third Affiliated Hospital of Qiqihar Medical College. Heilongjiang 161000, China? 　　【Abstract】 Objective To investigate the expression of cyclooxygenase-2(COX-2) and Bax protein in non-small cell lung cancer(NSCLC) ,and its clinical significance and correlations of COX-2 and Bax.Methods Samples were obtained from 57 cases NSCLC tissues(including adenocarcinoma and squamous cell carcinoma)，15 sampls nomal lung tissues and 57 samples Lymphoid tissue. The expression level of COX-2 protein and Bax protein in these samples were examined by immunohistochemistry. The relationship between COX-2 and Bax were analyzed by positive percentage. Results The expression of COX-2 protein in NSCLC tissues is higher than nomal tissue which were obained nearly (P0.05); The expression of Bax protein in NSCLC tissues was lower than nomal tissue which were obained neraly (P0.05).there was relationship between COX-2 and Bax(P3 cm)的正常组织］，组织离体后去除坏死或出血组织制备石蜡切片，标本均经１０％甲醛溶液固定，石蜡包埋。? 　　1．2 实验方法? 　　1．2．1 HE染色 石蜡标本4 μm连续切片，60℃烤片2 h。二甲苯脱蜡，经梯度酒精至水，苏木素染色细胞核，清洗，盐酸酒精分化，返蓝，伊红染细胞浆，梯度酒精脱伊红，二甲苯透明，中性树胶封片。? 　　1．2．2 免疫组织化学法 兔抗人COX-2免疫组化单克隆抗体、鼠抗人Bax免疫组化单克隆抗体、链霉卵白素过氧化酶(S-P)免疫组织化学试剂盒均购自于福州迈新生物技术开发有限公司。操作按S-P免疫组织化学???剂盒说明书进行, 操作步骤如下：石蜡切片常规脱蜡至水,内源性过氧化物酶阻断10 min,微波抗原修复,一抗孵育过夜(4℃),二抗孵育20 min(室温),链霉菌抗生物素蛋白过氧化物酶孵育30 min(室温),DAB显色5～10 min,苏木素复染后,脱水,透明,封片，光镜下观察。取阳性对照片同步操作作为阳性对照；用PBS代替一抗作为阴性对照。结果判定: 选择5个有代表性高倍视野,计算阳性细胞数，阳性细胞数≥31%为染色阳性,2检验。P＜0.05为差异有统计学意义。? 　　2 结果? 　　2．1 COX-2的检测结果? 　　2．1．1 COX-2在正常肺组织中及NSCLC中的表达 免疫组织化学结果 COX-2在57例NSCLC组织中表达率