FAT对K562A02细胞内GSH含量影响.docVIP

FAT对K562A02细胞内GSH含量影响 　　[摘要] 目的：探讨FAT对K562/A02细胞内谷胱甘肽（GSH）含量的影响，及其对白血病细胞代谢解毒系统介导的多药耐药（MDR）的逆转效果。方法：采用生化DTNB法测定细胞内谷胱甘肽水平。结果：K562/A02细胞内GSH含量（231.54 μmol/106细胞）高于K562细胞内GSH含量（58.03 μmol/106细胞），为K562细胞的3.99倍；VRP（10 μmol/L）、FAT（0.01、0.02、0.04、0.08 mg/ml）作用48 h后，K562/A02细胞内GSH含量从231.54 μmol/106细胞分别减少到96.95 μmol/106细胞、212.89 μmol/106细胞、161.00 μmol/106细胞、122.35 μmol/106细胞。结论：细胞内GSH含量增加是K562/A02细胞产生MDR的机制之一；FAT降低K562/A02细胞内GSH的含量是FAT逆转MDR的机制之一。 　　[关键词] 白血病；多药耐药；FAT；谷胱甘肽 　　[中图分类号] R-33 [文献标识码] A [文章编号] 1674-4721（2012）01（b）-005-02 　　 　　Effect of FAT on K562/A02 intracellular GSH concentration 　　ZHANG Yuxia1, YONG Guoxin1, LIANG Qiong2 　　1.Department of Technology Application, Hainan Technology and Business College in Hainan Province, Haikou 570203, China; 2.Department of Engineering Technology, Haikou College of Economics in Hainan Province, Haikou 570203, China 　　[Abstract] Objective: To investigate the impact of FAT on K562/A02 intracellular glutathione (GSH) concentration, and leukemia cell metabolism system of detoxification mediated drug-resistant (MDR) reversal effect. Methods: Intracellular GSH concentration was examined by biochemical DTNB method. Results: Intracellular GSH concentration in K562/A02 cell line (231.54 μmol/106 cells) was higher than that in K562 cell line (58.03 μmol/106 cells), and 3.99-fold greater in K562/A02 cell line compared with in K562 cell line. Intracellular GSH levels in K562/A02 cell line treated with VRP (10 μmol/L), FAT (0.02, 0.04, 0.08 mg/ml) for 48 hours (96.95 μmol/106 cells, 212.89 μmol/106 cells, 161.00 μmol/106 cells,122.35 μmol/106 cells respectively) was decreased than that in K562/A02 cell line without FAT (231.54 μmol/106 cells). The intracellular GSH concentration in K562/A02 cell line was higher than that in K562 cell line. Conclusion: The intracellular GSH content increase in K562/A02 cells to produce MDR mechanism of K562/A02. One of the mechanisms of MDR reversal by FAT is decrease of intracellular GSH levels on K