RP-HPLC测定拉莫三嗪和苯巴比妥血药浓度.docVIP

RP-HPLC测定拉莫三嗪和苯巴比妥血药浓度 　　[摘要] 目的:建立RP-HPLC法同时测定人血清中抗癫痫药拉莫三嗪和苯巴比妥浓度的方法。方法:采用Agilent Zorbax XDB-C18(4.6 mm×150 mm,5 μm)色谱柱;流动相:0.1%乙酸-乙腈,梯度洗脱;检测波长:240 nm;流速:1.0 ml/min。结果:拉莫三嗪和苯巴比妥的血药浓度分别在0.02～1.00 μg、0.02～3.00 μg范围内与峰面积呈良好的线性关系,平均回收率分别为97.62%和97.96%,拉莫三嗪2.0、20.0、40.0 μg/ml日内精密度(RSD,n=5)分别为4.3%、3.9%和2.9%,日间精密度(RSD,n=5)分别为5.1%、5.4%%和3.9%;苯巴比妥5.0、20.0和50.0 μg/ml日内精密度(RSD,n=5)分别为3.5%、4.2%和3.6%,日间精密度(RSD,n=5)分别为5.0%、4.7%和4.2%。结论:本方法简便、准确、重现性好,可用于临床上常规监测拉莫三嗪和苯巴比妥的血药浓度。 　　[关键词] 拉莫三嗪;苯巴比妥;血药浓度;梯度洗脱;反相高效液相法 　　[中图分类号] R927.2[文献标识码] B[文章编号] 1673-7210(2010)03(b)-039-03 　　 　　Content determination of Lamotrigine and Phenobarbital in human plasma by RP-HPLC 　　DU Jun, LI Yijun* 　　(Department of Pharmacy, Peoples Hospital of Jingshi, Jingshi415400, China) 　　[Abstract] Objective: To establish a method for the content determination of lamotrigine and phenobarbital in human plasma by RP-HPLC. Methods: The Agilent Zorbax XDB-C18 (4.6 mm×150 mm, 5 μm) column was used. The mobile phases consisted of 0.1% acetic acid and acetonitrile, gradient elution. The flow rate was 1.0 ml/min and the detection wavelength was at 240 nm. Results: The linear ranges of Diphenhydramine Hydrochloride were at 0.02-1.00 μg and 0.02-3.00 μg, respectively. Lamotrigine presented a good linear relationship in the range of 1.00-50.0 μg/ml (r=0.999 1); phenobarbital in the range of 1.00-150.0 μg/ml (r=0.999 0). The intra-day RSD (n=5) of 2.0, 20.0 and 40.0 μg/ml of lamotrigine were 4.3%, 3.9% and 2.9%; the inter-day RSD (n=5) were 5.1%, 5.4% and 3.9%. The intra-day RSD of 5.0, 20.0 and 50.0 μg/ml of phenobarbital were 3.5%, 4.2% and 3.6% (n=5); the inter-day RSD were 5.0%, 4.7% and 4.2% (n=5),respectively. The recovery rates (n=5) of lamotrigine and phenobarbital were 97.62% and 97.96%, respectively.Conclusion: The method is fast, reliable, accurate and can be used in monitoring plasma levels of lamotrigine and phenobarbital in clinic. 　　[Key words] Lamotrigine; Phenobarbital; Serum level of drug; Gradient