乌司他丁对脓毒症大鼠肺微血管通透性及肺组织VEGF表达影响.docVIP

乌司他丁对脓毒症大鼠肺微血管通透性及肺组织VEGF表达影响 　　【摘要】 目的 观察乌司他丁注射液对脓毒症大鼠肺微血管通透性及肺组织血管内皮生长因子(VEGF)表达的影响,探讨乌司他丁对脓毒症大鼠肺保护的作用机制。方法 30只健康的雄性SD大鼠随机分为3组:假手术组(A组)、阳性对照组(B组)、乌司他丁治疗组(C组),每组10例。除假手术组外,其他组均施行盲肠结扎穿孔术(CLP)复制大鼠脓毒症模型,治疗组同时给予乌司他丁腹腔注射(100 ku/kg),术后4 h随机处死大鼠5只取组织标本,剩余5只大鼠经颈外静脉注射伊文思蓝观察肺微血管通透性。分别采用逆转录聚合酶链反应(RT-PCR)法和酶联免疫吸附(ELISA)法检测肺组织中VEGF的表达。结果 B组肺微血管通透性及肺组织VEGF表达水平明显高于A、C组;C组肺微血管通透性及肺组织VEGF表达水平高于A组(P 　　【关键词】乌司他丁注射液;脓毒症;肺微血管通透性;VEGF 　　 　　Effect of Ulinastatin injection on the permeability of pulmonary microvascular and expression of vascular endothelial growth factor in lung tissue of sepsis in rats 　　CAI Shi-xia, DENG Lie-hua,TONG Lin, et al.Intensive Care Unit, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001,China 　　 　　【Abstract】 Objective To observe the permeability of pulmonary microvascular and expression of vascular endothelial growth factor in septic rats treated with Ulinastatin injection, and to investigate the action mechanism of Ulinastatin in pulmonary function protect.Methods A total of 30 healthy male SD rats were randomly divided into three groups, and each group had 10 rats,Group A received sham operation and the others underwent cecal ligation and puncture (CLP) operation.All the rats were accepted intraperitoneal injection of normal saline ( group A,B) or Ulinastatin (100 ku/kg).After four hours, Lung tissue express of VEGF in all rats were detected by reverse transcription-polymerase chain reaction(RT-PCR) and Enzyme-linked immunosorbent assay(ELISA) methods respectively and the permeability of pulmonary vascular were observed by injecting Evans Blue into jugular vein of rats.Results The permeability of pulmonary microvascular and the expression levels of VEGF in group B were significantly higher than those in group A and C,which in group C were significantly higher than that in group A.Conclusions Ulinastatinis a reliable protection agent for septic induce acute lung injury, and its mechanism of action may be involved in the inhibition of