共生食用菌松茸菌丝体液体发酵研究.docVIP

共生食用菌松茸菌丝体液体发酵研究 　　摘要：目的研究松茸菌丝体液体发酵方法和最佳条件。方法经RAPD-PCR 分析确认松茸菌丝体菌种，研究松茸液体深层发酵过程中菌丝体的生物量、残糖浓度和pH随时间变化的过程；比较不同种龄对菌丝体积累的影响，确立最佳接种种龄；比较不同初始糖浓度发酵过程的结果，运用中间补糖的方法，确定最优的补糖时间。结果最佳接种种龄为6 d；初始糖浓度较低有利于缩短松茸种子液生长的延迟期，较高则可使菌丝体在较长时间保持生长并获得较高的生物量产出。d 12 为最优补糖时间，在摇瓶体系中培养24 d最终得到12.77 g/L的菌丝体生物量。结论最佳培养条件下，采用中间补糖法适合液体发酵过程中松茸菌丝体的积累。 　　关键词：松茸；菌丝体；种龄；补料培养；液体发酵 　　中图分类号：TS201.3文献标识码：A文章编号：1672-979X(2007)05-0001-04 　　 　　Submerged Culture of Mycelium of Ectomycorrhizal Basidiomycete Tricholoma matsutake 　　LI Yun1, ZENG Dong-fang2 　　(1. Department of Biology, Normal University of Hanshan, Chaozhou 521041, China； 　　2. School of Chemical Engineering ＆ Pharmacy, Wuhan Institute of Technology, Wuhan 430074, China ) 　　Abstract：Objective To investigate the method and optimum conditions of Tricholoma matsutake mycelium fermentation.Methods Dynamic changes of biomass, residual glucose concentration and pH in submerged culture was investigated with Tricholoma matsutake stain, which was isolated and identified by RAPD-PCR method. By comparing the effect of different inoculum ages on biomass, the optimum inoculum age was determined. Based on comparing the effect of different initial glucose concentrations on dynamic changes of fermentation, the optimum time of feeding glucose was established by carrying out the feeding culture strategy in submerged culture. ResultsThe optimum inoculum age was 6 days. The lower initial glucose concentration obviously availed to shorten the lag 　　phase of the growing of mycelium. And the higher initial glucose concentration made the mycelium maintain growth for a long period of time and finally reached a higher mycelium yield. The d 12 was confirmed as the optimum feeding time. By applying the feeding culture strategy, 12.77g/L mycelium biomass was obtained on day 24 in flask system. Conclusion The method and optimum conditions is feasible for accumulating the mycelium of Tricholoma matsutake in submerged culture. 　　Key words：Tricholoma matsutake; mycelium; ino