雷米普利对单侧输尿管梗阻大鼠肾组织骨形态蛋白-表达影响.docxVIP

华中科技大学硕士学位论文Effection of Ramipril on the renalexpression of bone morphogeneticprotein-7: an experimental studyin unilateral ureteral obstructionratsDepartment of nephrology, Tongji Hospital, Tongji Medical College,Huazhong University of Science and TechnologySupervisor:Professor Lv YongmanPostgraduate: Du FanAbstractObjective:To investigate the effection of Ramipril on the expression of bonemorphogenetic protein-7 of renal interstitial following unilateral ureteral obstruction.Methods:Adult male SD rats were randomly divided into five groups: Shamoperated group (sham group), UUO vehicle group (UUO-1 group and UUO-2 group), UUOtreatedwith Ramipril 10 mg·kg-1·d-1 (ACEI-1 group and ACEI-2 group), the rats inACEI-1 group were treated with Ramipril one week after unilateral ureteral obstruction,ACEI-2 group were treated with Ramipril right after unilateral ureteral obstruction .On the1 week after operation , the obstructed kidneys of rats in UUO-1 group were taken out to bemeasured by Masson stain and RT-PCR method, the kidneys of the other groups were takenout on the 2 week after operation. The degree of tubulointerstitial damage was scored.3华中科技大学硕士学位论文Results:In comparison with UUO-2 group, renal interstitial expression of bonemorphogenetic protein-7 were significantly increased in ACEI-2 group, the degree oftubulointerstitial damage were reduced .On the base of renal tubulointerstitial fibrosis,interstitial expression of bone morphogenetic protein-7 were increased and the degree oftubulointerstitial damage were reduced in ACEI-1 group.Conclusions:Ramipril may maintain expression of bone morphogenetic protein-7on the course of renal tubulointerstitial fibrosis,slow the advance of the tubulointerstitial .Key Words:Ramipril;renal interstitium fibrosis;bone morphogeneticprotein-74华中科技大学硕士学位论文前言肾间质纤维化几乎是所有慢性肾脏疾病发展的最终结果,与慢性肾衰竭进展密切相关,并被认为比肾小球病变更能反应慢性肾衰竭的进展速率及预后。有研究表明小管-间质纤维化组织学分级与肾小球硬化的组织学分级相比更能反映肾功能的丧失程度[1]。已有的研究表明,肾小管上皮细胞肌成纤维细胞转分化(epithelial myofibroblasttransdifferentiat