高住低练对线粒体钙转运功能及能量代谢作用.docVIP

高住低练对线粒体钙转运功能及能量代谢作用 　　摘 要： 目的：探讨高住低练对线粒体钙转运功能及能量代谢的影响，为运动与低氧适应 机制的研究和高原训练方案的制定提供理论依据。方法：采用荧光技术和生物发光技术分别 测定心肌、肝脏和骨骼肌线粒体Ca?2＋?浓度和心肌线粒体ATP含量，并用血乳酸分析仪 和血糖仪分别测定血乳酸和血糖浓度。结果：急性低氧应激使线粒体游离Ca?2＋?浓度 和ATP含量下降，血乳酸明显上升；经过高住低练运动与低氧适应后线粒体游离Ca?2＋? 浓度和ATP含量呈上升 趋势，血乳酸上升程度减低，提示依赖糖酵解产能降低，能量代谢产生了适应。结论：高住 低练能使线粒体钙转运功能加强，能量代谢作用得到改善。 　　关键词：高住低练；线粒体；钙转运；能量代谢 　　中图分类号：G804.7文献标识码：A文章编 号：1007-3612(2011)03-0056-03 　　 　　Effects of HiLo Training on the Function of Mitochondria CalciumTransports and Energy Metabolism 　　HUANG Li ying，WENG Xi quan，LIN Wen tao 　　(Exercise Biochemistry Section， Guangzhou Institute of Physica l Education，Guangzhou 510500，Guangdong China) 　　Abstract: Aim: The effects of HiLo training on the function of mitochondria calcium transp orts and energy metabolism were investigated， so that the theory of study on th e mechanisms of hypoxic acclimatization and project of high altitude training isprovided. Methods: The Ca?2＋?concentration of mitochondria in the heart，liver and skeletal muscle tissue and ATP content were assessed by fluorescenceand bioluminescence techniques， and blood lactate and blood glucose conce ntration were measured by blood lactate analyzer and glucose meters. Results: F ree Ca?2＋?concentration， ATP content of mitochondria decreased after hypox ic str ess and blood lactic acid increased; Free Ca?2＋?concentration， ATP contentof mi tochondria increased after 4 week hypoxic acclimatization at altitude 3 000 m a nd significantly after hypoxic training acclimatization， blood lactic acid incr easing a little shows that it depending on energy of glycolytic decreased， ener gy metabolism and energy metabolism developed adaptation. Conclusion: The funct ion of mitochondria calcium transports increased and energy metabolism was impro ved after living high training low. 　　Key words: Living high training low; mitochondria; calcium transports;energy metabolism 　　 　　高住低练的高原训练法是运动员训练在低海拔，同时休息在高海拔缺氧低压环境以便使 得机体发生缺氧习服现象，其优点是不会明显降低运动训练的强度，从而提高运动员的运动 能力。研究表明，高住低练能显