高危型人乳头瘤病毒感染与肺癌关系.docVIP

高危型人乳头瘤病毒感染与肺癌关系 　　【摘要】 目的 探讨豫北地区肺癌中高危型人乳头瘤病毒(HPVs)感染情况及分型，为初步明确HPVs感染与肺癌的关系提供依据。方法 收集新鲜肺癌标本63例，组织离体后分为两部分处理：一部分用于HE染色，以明确病变性质；另一部分用于提取基因组DNA。采用聚合酶链反应(PCR)方法检测肺癌组织中高危型HPVs的感染情况及分型，并分析相关高危型HPVs感染与肺癌组织学类型的关系。结果 经病理检验证实，收集的63例肺癌标本包括非小细胞肺癌40例，小细胞肺癌23例。在63例肺癌中，HPV16感染的阳性率为68．25%(43/63)，其中65．12%(28/43)为HPV16单独感染，34．88%(15/43)为HPV16与HPV59混合感染。HPV59感染的阳性率为23．81%(15/63)，均为与HPV16混合感染。未检测到HPV18、31、33、58和52感染的病例。在非小细胞肺癌和小细胞肺癌中，HPV16阳性率差异有统计学意义(χ2=6．977，P2=0．086，P0．05)。结论 高危型HPVs，特别是HPV16感染与肺癌存在密切的关系，且与其他类型HPVs的混合感染值得重视。? 　　【关键词】 　　人乳头瘤病毒；肺癌；组织学类型 　　 　　The relationship between the infection of high risk-human papillomavirues and lung cancer 　　 　　WEI Jun-fei，QIAN Xin-lai.The First Rongkang Hospital,DepartmentofPathology，XinxiangMedicalUniversity,Henan Xinxiang 453003,China 　　? 　　【Abstract】 Objective To investigate the relationship between the infection of high risk-human papillomavirues and lung cancer in north of Henan province,and preliminarily definitize the role of high risk-HPVs in carcinogenesis of lung cancer.Methods 63 fresh lung cancers were collected.Every specimen was divided into two parts:one part was stained by HE.Histopathological assessment was performed on paraffin sections.The second part was used to extract genomic DNA.The infection rate and typing of high risk-HPVs were detected in lung cancer by PCR,and the relation between relative high risk-HPVs and the tissue classifications of lung cancer was also analyzed.Results The results of pathological diagnose showed 63 fresh specimens collected in the study were composed of 40 cases of non-small cell lung cancer(NSCLC)and 23 cases of small cell lung cancer(SCLC).The positive rate of HPV16 was 68．25%(43/63)in lung cancer.Among HPV16 infection in lung cancer,the HPV16 infection alone and HPV16 and HPV59 double infection were 65．12(28/43)and 34．88%(15/43),respectively.Furthermore,the positive rate of HPV59 was 23．81%(15/63)in lung cancer.Among HPV59 infection in lung cancer,100%(15/15)was mixed infection of