双壳贝类线粒体基因组结构比较Comparison of mitochondrial ge.pdfVIP

HEREDITAS (Beijing) 2009 年 11 月, 31(11): ISSN 0253-9772 研究报告 双壳贝类线粒体基因组结构的比较 1, 2 1 1 1 1, 2 1 宋文涛 ,高祥刚 ,李云峰 ，刘卫东 ,刘 莹 ，赫崇波 1. 辽宁省海洋水产科学研究院，辽宁省海洋水产分子生物学重点实验室，大连116023； 2. 辽宁师范大学 生命科学学院，大连 116029 摘要:利用比较基因组学和生物信息学方法，比较分析了已登录到GenBank 中的14种海产双壳贝类和2种淡水 双壳贝类的线粒体基因组的结构特征。结果发现，双壳贝类线粒体的基因组结构、基因排列顺序均互不相 同；不同目、科和属之间线粒体基因组的大小、基因排列方式以及基因种类也存在明显的差异，尤其是基 因排列方式没有明显的规律。对16种双壳贝类的线粒体基因组全序列、编码基因序列进行系统分析，分别 得到了不同的聚类结果，即用基因组全序列聚类时，16种贝类的聚类结果与传统的形态学分类地位基本相 同；而将16种贝类的所有蛋白质编码基因和2个rRNA基因按照一致顺序排列起来进行聚类时，所得的系统 分类情况与这些贝类传统的形态学分类地位相差较大。 关键词：双壳贝类；线粒体全基因组；基因组结构；多态性 Comparison of mitochondrial genomes of Bivalves 1, 2 1 1 1 1, 2 1 SONG Wen-Tao , GAO Xiang-Gang , LI Yun-Feng , LIU Wei-Dong , LIU Ying , HE Chong-Bo 1. Liaoning Ocean and Fisheries Science Research Institute, Liaoning Key Laboratory of Marine Fishery Molecular Biology, Dalian 116023, China; 2. College of Life Sciences, Liaoning Normal University, Dalian 116029, China Abstract: The structure and organization of mitochondrial genomes of 14 marine bivalves and two freshwater bivalves were analyzed using comparative genomics and bioinformatics methods. The results showed that the organization and gene order of the mitochondrial genomes of these bivalve species studied were different from each other. The size, organization, gene numbers, and gene order of mitochondrial genomes in bivalves at different taxa were different. Phylogenetic analysis using the whole mitochondrial genomes and all the coding genes showed different results- phylogenetic analysis conducted using the whole mitochondrial genomes was consistent with the existing classification and phylogenetic analysis conducted using