哮喘大鼠TGF-β1Smad3信号通路中黄芪与激素调控对比研究.docVIP

哮喘大鼠TGF-β1Smad3信号通路中黄芪与激素调控对比研究 　　摘 要：目的：观察黄芪、糖皮质激素对哮喘大鼠TGF-β1/Smad3信号通路及哮喘气道重塑的对比调控作用。方法 SPF级雄性SD大鼠50只随机分为5组，分别为对照组（A组）、哮喘组（B组）、布地奈德组（C组）、地塞米松组（D组）、黄芪组（F组），每组10只。用卵白蛋白(OVA)致敏与激发建立大鼠哮喘模型。应用图像分析技术测定肺内支气管壁厚度（Wat）及平滑肌厚度（Wam）等重塑指标；免疫组化法检测肺组织TGF-β1、P-Smad3蛋白的表达；酶联免疫吸附试验（ELISA）法测定血清和支气管肺泡灌洗液（BALF）中TGF-β1的浓度。结果：干预组大鼠Wat、Wam较哮喘组显著性降低(均P 　　关键词：哮喘；气道重塑；转化生长因子-β1；Smad3；糖皮质激素；黄芪 　　 中图分类号：R256.12文献标识码：A文章编号：1673-7717(2011)12-2645-04 　　Regulation of Glucocorticoids and Radix Astragali Comparison study of 　　TGF-β1/Smad3 Signal Pathway on Airway Remodelling in Asthma Rats 　　DAI Huan,ZHANG Weixi,HE Xiaoliang,ZHAO Ruixue,FANG Li,LI Changchong 　　(Department of Pediatric Respiratory,Yuying Childrens Hospital,Wenzhou Medical College,Wenzhou 325027,Zhejiang,China) 　　________________________________________ 　　收稿日期：2011-07-09 　　基金项目：国家自然科学基金资助项目 　　作者简介：戴欢（1977－），女，浙江温州人，医师，硕士，研究方向：支气管哮喘的发病机制。 　　通讯作者：李昌荣（1956-），男，浙江温州人，教授，博士研究生导师，研究方向：支气管哮喘的发病机制。Abstract:Objective:The regulation of Glucocorticoids and Radix Astragali (RA) toTGF-β1/ Smad3 signal pathway and asthma airway remodeling was observed.Methods：In the experiment, 50 male Sprague－Dawlay(SD) rats, grade SPF were needed. Asthmatic airway remodelling model rats were sensitized with ovalbumin and AL(OH)3,and repeatedly exposed to aerosolized ovalbumin. According to the repeated time course of challenge, rats were randomly divided into the control group(group A), asthma group(group B) and treated group with BUD(group C)，treaded group with DXM(group D)，treated group with RA (group F).Every group has ten rats. The total brochial wall thickness (Wat) and the airway smooth muscle thickness (Wam) were measured by image analysis system.The protein expressions of TGF-β1 and P-Smad3 in the lung tissues were detected by immunohistochemistry technique.The concentrations of TGF-β1 in serum and BALF were tested by sandwich ELISA.Results:Compared with group B,Wat and Wam of treated groups were significantly decreased(P