反枝苋种子萌发和幼苗生长对盐度碱度和温度响应.docVIP

反枝苋种子萌发和幼苗生长对盐度、碱度和温度的响应 【摘要】：【关键词】： Amaranthus seed germination and seedling growth of salinity, alkalinity and temperature response HaYaling (School of Life science and Chemistry,TianShui Normal University,Tianshui741000,China) 【Abstract】：in order to Amaranthus seeds as the experimental materials, study of seed germination and seedling growth of different salinity, alkalinity and temperature response, the results showed that: different concentrations of salt, alkali stress, 5, 15, 25℃ under conditions of seed germination rate and seedling growth of no significant difference, the temperature is not inhibited seed germination the main limiting factor. When the temperature is high up to 35℃ , seeds to germinate properly, description of Amaranthus seeds on high temperature stress resistance is not strong. The same temperature condition, the germination rate of seeds and buds with salinity, alkalinity increased shows decreasing trend. The same concentration of alkali stress than salt stress showed stronger inhibition effect. The variance analysis showed that: salt, temperature and alkalinity interaction does not alter the salt stress, alkaline stress on Amaranthus seed germination and seedling growth of.。 【Key words】：Amaranthus retroflexus, salinity, temperature, germination, seedling growth 反枝苋，又名苋菜，野苋菜，茵茵菜（东北），苋科，苋属。在世界许多地方被列为恶性杂草。生于山坡，路旁，旷野，荒地，田边，沟旁，河岸等处。反枝苋的嫩叶可食用，也可作饲料，种子及全株均可入药，治疗腹、痢疾、痔疮肿痛等。Marten和Anderson报道反枝苋对绵羊的适口性和燕麦一样，并且其营养成分含量和消化率接近苜蓿，是一种不错的饲料[1]。对自然界植物资源进行充分利用，研究杂草的饲料学价值，对畜牧业生产具有积极意义。本文以反枝苋种子为材料，对它在不同温度、盐溶液、碱溶液中的萌发及其幼苗生长进行了研究，旨在探明种子对温度、盐度、4溶液浸泡15分钟，以解除休眠。然后用蒸馏水将种子清洗3～5次，每次3min.以除去KMnO4溶液。种子的萌发实验30粒1组。3次重复。 1.2 试剂 蒸馏水，氯化钠，碳酸钠 1.3 仪器设备 培养皿，恒温箱，直尺 1.4 试验设计 1.4.1 不同温度，不同浓度盐溶液对反枝苋种子萌发的影响 NaCl溶液分别配制成质量浓度为0（ck）、0.6、1.2、2.4、3.6、4.8g/L浓度的溶液。在洗净、烘干的培养皿中放入蒸馏水湿润的滤纸。将种子置于培养皿中，30粒1组。向培养皿中分别倒入不同质量浓度的NaCl溶液，每个浓度梯度设3次重复。用蒸馏水作对照。将培养皿分别放入5、15、25℃的恒温培养箱中进行培养，观察记录发芽种子并补充蒸发的溶液。7d后，检测种子发芽个数，并测其芽长。 1.4.2 不同温度，不同浓度碱溶液对反枝苋种子萌发的影响 Na2CO3溶液分别配制成质量浓度为0（ck）、0.1、0.3、0.6、0.8、1.