Transport of Cytoplasmically Syntheized Proteins into 运输到细胞质合成蛋白质.pptVIP

Post-Translational Events I Translocation of Newly Synthesized Proteins into Membranous Organelles Locations of Protein Synthesis ER Associated Ribosomes proteins translocate co-translationally into ER lumen subsequent processing transport through Golgi for secretory vesicles and lysosomes Cytoplasmic Ribosomes proteins translocated post-translationally into membranous organelles (except Golgi, ER and lysosome) Transport of Cytoplasmically Synthesized Proteins Import into organelles (transmembrane and lumenal) Mitochondrial import Chloroplast import Peroxisome import Translocation across plasma membrane Insertion in cytoplasmic face of plasma membrane Signal Sequences Portions of protein sequence Frequently N-terminal, C-terminal Occasionally located within Often cleaved following transport event Signal Recognition Particles/Complexes protein-translocation channels escort (chaperone) proteins Mitochondrial Protein Import Four Destinations Outer membrane Innermembranous space Inner membrane Matrix TOMs Translocase of the outer mitochondrial membrane TIMs Translocase of the inner mitochondrial membrane Tiny TIMs escorts of the innermembranous space TOM TIM Function Tiny TIMs and TIM22 TOC TIC Peroxisome Import Peroxisome Formation Translocation of ER-Associated Ribosome Synthesized Proteins Docking of Ribosomes to ER Signal Recognition Particle Ribonucleoprotein complex Similar to bacterial SRP Translocation of Various Classes of ER Proteins * Signal sequence for recognition by PEX5 -SKL-COOH Signal peptides typically 10-20 residues – shorter than those on cytostolically synthesized proteins Ribosome binding Signal peptide binding translocation *