水蛭素活性因子抗癌作用实验研究.docVIP

水蛭素活性因子抗癌作用实验研究 　　[摘要] 目的 观察水蛭素活性因子对H22肝癌瘤细胞、S180肉瘤的抑制作用，为临床用药提供理论依据。 方法 取小鼠48只，每只小鼠左前腋下接种H22肝癌瘤细胞0.2 mL，随机分为4组，每组12只，分成模型对照组、5-FU组、水蛭素活性因子大剂量组（水蛭素活性因子2.6 g/kg）和水蛭素活性因子小剂量组（水蛭素活性因子1.3 g/kg）；另取小鼠44只，每只小鼠左前腋下接种S180肉瘤细胞0.2 mL，随机分为4组，每组11只，分成模型对照组、5-FU组、水蛭素活性因子大剂量组（水蛭素活性因子2.6 g/kg）和水蛭素活性因子小剂量组（水蛭素活性因子1.3 g/kg）；连续给药14 d，比较各组肿瘤生长情况，并计算抑瘤率。 结果 ①水蛭素活性因子对H22肝癌实体瘤的影响：5-FU组[瘤重（0.78±0.41）g]、水蛭素活性因子大剂量组[瘤重（0.99±0.48）g]、水蛭素活性因子小剂量组[瘤重（1.15±0.50）g]对H22肝癌抑瘤率分别为58.73%、47.62%、39.15%，与模型对照组相比，差异均有统计学意义（P 0.05）。③所有5-FU组小鼠在给药第9天开始出现精神萎靡、蜷缩少动、脱毛、食欲减退等，而水蛭素活性因子组小鼠未发生此现象。 结论 水蛭素活性因子对H22肝癌瘤细胞和S180肉瘤均有较好的抑制作用，且水蛭素因子无明显副作用。 　　[关键词] 水蛭素活性因子；H22肝癌瘤细胞；S180肉瘤；副作用 　　[中图分类号] R285.5 [文献标识码] A [文章编号] 1673-7210（2014）01（b）-0008-03 　　Experimental study of anti-tumor effect of hirudin active factor 　　CUI Meiyue WANG Xingfu YAN Huailing 　　Department of Internal Medicine， the Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine， Shandong Province， Jinan 250001， China 　　[Abstract] Objective To observe the anti-tumor effect of hirudin active factor on H22 liver tumor cells and S180 sarcoma， thus to provide theoretical basis for clinical pharmacy. Methods 48 mice were selected， 0.2 mL H22 liver tumor cells inoculated subcutaneously， divided into 4 groups， each group had 12 mice. Four groups were named as control group， 5-FU group， high-dose hirudin group （hirudin active factor 2.6 g/kg）， low-dose hirudin group （hirudin active factor 1.3 g/kg）. Another 44 mice were selected， 0.2 mL S180 sarcoma cell inoculated subcutaneously， divided into 4 groups， each group had 11 mice. Four groups were named as control group， 5-FU group， high-dose hirudin group （hirudin active factor 2.6 g/kg）， low-dose hirudin group （hirudin active factor 1.3 g/kg）. All mice were treated by saline， 5-FU group， high-dose hirudin， low-dose hirudin respectively for 14 days daily. The growth of carcinoma cell were compared， and the anti-carcinoma rate was calculated. Results ①The effect of hirudin active f