糖尿病大鼠肺脏微循环通透性变化及其意义.docVIP

糖尿病大鼠肺脏微循环通透性变化及其意义 　　[摘要] 目的 观察实验性糖尿病大鼠肺脏微血管通透性的变化，为临床提供糖尿病肺病的形态学依据。 方法 雄性SD大鼠共60只，体重（220±20）g，按65 mg/kg的剂量一次性腹腔注射链脲菌素（STZ）来制备糖尿病大鼠模型，在成模即刻（0周）、成模后2、4、8、12周时分别随机抽取12只，通过干湿重法、尾静脉注入伊文思蓝（EB）并心脏灌注后在分光光度计下测肺组织OD值以及荧光显微镜下测其荧光量来了解肺脏微血管的通透性。另随机抽取12只大鼠作为正常组。 结果 ①与正常组比较，0周组、2周组糖尿病大鼠肺组织的干湿重稍有增加，但差异无统计学意义（P 0.05）。而4周组、8周组和12周组糖尿病大鼠出现了组织干湿重明显增加，差异有统计学意义（P 0.05）。从4周开始，糖尿病大鼠出现肺组织EB含量明显增加，差异有统计学意义（P 　　[关键词] 糖尿病；糖尿病肺病；微循环障碍；伊文思蓝；通透性 　　[中图分类号] R587.1 [文献标识码] A [文章编号] 1673-7210（2014）01（c）-0023-04 　　Changes in microvascular permeability of lung in experimental diabetic rats and its significance 　　PENG Liyuan1 WANG Hailan1 ZHAI Fanglong1 XIE Zaiming1 WANG Mingjun2 　　1.Affiliated Shenzhen Longgang Central Hospital of Zunyi Medical College， Guangdong Province， Shenzhen 518116， China； 2.Shenzhen Childrens Hospital， Guangdong Province， Shenzhen 518000， China. 　　[Abstract] Objective To observe the changes of microvascular permeability of lung in experimental diabetic rats， and to offer clinical morphologic data for diabetic pulmonary disease. Methods 60 male SD rats with （220 ±20） g were selected and injected with Streptozotocin （STZ） intraperitoneally at a dose of 65 mg/kg body weight to make experimental DM rat models. Then respectively， 12 of them were randomly selected at 0， 2， 4， 8， 12 weeks to measure their dry weight and wet weight of lung tissues. Also， the OD values were observed through spectrophotometer and the amount of fluorescence by a fluorescence microscope after the rats were injected Evans blue （EB） into tail vein. Another 12 rats were randomly selected as normal group. Results ①The difference of lung tissue wet-dry weight among normal group and 0 week group， 2 weeks group was not statistically significant ???P 0.05）. The lung tissue wet-dry weight in 4 weeks group， 8 weeks group， 12 weeks group were all higher than those in normal group， the differences were statistically significant （P 0.05）. The lung tissue EB content in 4 weeks group， 8 weeks group， 12 week