脓毒症脑病大鼠血清和脑组织S100β变化.docVIP

脓毒症脑病大鼠血清和脑组织S100β变化 　　【摘要】目的 探讨S100β在脓毒症脑病大鼠体内的变化及意义。方法 30只SD大鼠安放脑电极后，18只建立盲肠结扎穿孔法诱发的脓毒症模型，余12只随机（随机数字法）分为正常组6只，假手术组6只，利用RM6240生理信号记录仪记录心率、血压、脑电图的变化，通过脑电图变化判断脓毒症脑病的发生，8 h后留取血清和脑组织标本，用双抗体夹心酶联免疫分析法检测血清和脑组织标本S100β的浓度。对各组心率、平均动脉压、神经反射评分、血清S100β、脑组织匀浆S100β及脑组织匀浆/血清S100β采用单向方差分析进行统计学分析。结果 18只脓毒症模型鼠死亡3只，余分为脓毒症非脑病组8只，脓毒症脑病组7只。脓毒症脑病组血清和脑组织匀浆S100β浓度明显高于其他各组（P 　　【关键词】S100β；脑电图；血脑屏障；脓毒症脑病；脓毒症；神经反射评分；生化标志物；盲肠结扎模型 　　The changes of S100β in serum and brain of rats with sepsis-associated encephalopathy Yao Bo，Ai Yuhang，Zhang Lina. Department of Central Intensive Care Unit，Xiangya Hospital，Central South University，Changsha 410008，China 　　Corresponding author：AI Yu-hang， Email： ayhicu1978@ 　　【Abstract】Objective To study the changes of S100βin serum and brain tissue of rats with sepsis-associated encephalopathy （SAE）. Methods After placement of pole plates of electroencephalogram （EEG） on the brain cortex， thirty SD rats were randomly（random number） divided into three groups post hoc： sepsis group in which rats were made to be sepsis models by cecal ligation and puncture （n=18）， normal group （n=6） and sham operation group （n=6）. The sepsis-associated encephalopathy was diagnosed with electroencephalogram taken by RM6240 physiological signal recorder. Meanwhile， heart rate and blood pressure were recorded. Eight hours later， serum and brain tissue of sacrificed rats were taken for measuring S100β. The results ware analyzed with one-way ANOVA.Results Of 18 sepsis rats， 3 were dead， 8 without SAE and 7 with SAE. The levels of S100βin serum and brain tissue of rats with SAE group were significantly higher than those in normal group and sham operation proup （P[1]、脑血管疾病[2]、心肺复苏后脑损伤[3]等疾病的辅助诊断，但其用于诊断脓毒症脑病的研究???对较少。本研究的目的为建立盲肠结扎穿孔（CLP法）诱发的大鼠脓毒症模型，通过脑电图判断脓毒症脑病发生，来探讨S100β在脓毒症脑病大鼠体内的变化及意义。 　　1 材料和方法 　　1.1 实验动物 　　健康清洁级（SPF级）成年SD雄性大鼠30只，体质量250～300 g，由中南大学动物学部提供。 　　1.2 主要药品和试剂 　　S100βELISA试剂盒购于长沙佳和生物科技有限责任公司；牙科磷酸锌水门汀（武汉大学口腔医学院口腔药物材料厂）。 　　1.3 主要仪器 　　脑立体定位仪、RM6240生物信号记录仪均由中南大学动物学部提供。