高迁移率族蛋白N2对人舌癌裸鼠移植瘤抑瘤作用研究.docVIP

高迁移率族蛋白N2对人舌癌裸鼠移植瘤抑瘤作用研究 　　[摘要] 目的 研究高迁移率族蛋白N2（HMGN2）对人舌癌裸鼠移植瘤生长的抑瘤作用。方法 裸鼠腋下注射接种舌鳞癌细胞Tca8113以建立裸鼠移植瘤模型，接种7 d成瘤后，设立阴性对照组、HMGN2蛋白组和阳性对照组，在瘤体周边分别注射含washingbuffer Ⅱ、HMGN2蛋白和顺铂的细胞培养基。观察各组裸鼠肿瘤的生长情况，经过4次给药后处死裸鼠，取出瘤块，称其质量计算抑瘤率，并行苏木精-伊红（HE）染色观察形态学变化。结果 成功建立舌癌裸鼠移植瘤模型。HMGN2蛋白组和阳性对照组肿瘤体积明显小于阴性对照组。裸鼠体重在整个实验过程 　　中并没有明显的变化。阴性对照组、HMGN2蛋白组、阳性对照组的肿瘤质量平均值分别为（0.38±0.19）、（0.21± 　　0.15）、（0.23±0.16）g，3组间的差异无统计学意义。HMGN2蛋白组和阳性对照组的抑瘤率分别为45.71%和39.44%。肿瘤经肉眼观察可见阴性对照组瘤块较大，HE染色可见HMGN2蛋白组和阳性对照组细胞坏死明显。结论 HMGN2蛋白可以明显抑制人舌癌裸鼠移植瘤的生长。 　　[关键词] 高迁移率族蛋白N2； 移植瘤； Tca8113细胞 　　[中图分类号] R 730.2 [文献标志码] A [doi] 10.7518/hxkq.2014.01.002 　　Inhibitory effects of high mobility group chromosomal protein N2 on human tongue carcinoma transplanted in nude mice Liu Xiqian， Dong Xiaoqian， Zhang Yonghong， Zhang Ping， Li Xiaoyu， Chen Sixiu， Feng Yun. （State Key Laboratory of Oral Diseases， West China Hospital of Stomatology， Sichuan University， Chengdu 610041， China） 　　[Abstract] Objective To evaluate the inhibitory effect of high mobility group chromosomal protein N2 （HMGN2） on human tongue carcinoma tumor in nude mice. Methods A transplantation tumor model in nude mice was constructed by injecting Tca8113 cells. After a week， negative control groups， masculine control groups， and HMGN2 groups were esta-blished. Cell culture of the three groups were separately injected with washing buffer Ⅱ， cis-dichlorodiamineplatinum （DDP）， and HMGN2 protein. The tumors were moved after four treatments， and then analyzed by hematoxylin-eosin （HE） staining. Results A transplanted tumor model was established successfully. The volumes of HMGN2 groups and masculine control groups were smaller than those of the negative groups. Mouse weight did not differ among the three groups. Average tumor weight of the negative group was （0.38±0.19） g， that of the HMGN2 group was （0.21±0.15） g， and that of the DDP group was （0.23±0.16） g. These factors indicated no statistically significant difference among the three groups. The tumor inhibitory