肝脏病研究方.pptxVIP

Coenzyme Q in pregnant women and rats with intrahepatic cholestasis;PPT;Background aims intrahepatic cholestasis of pregnancy is a high-risk liver disease given the eventual deleterious consequences that may occur in the foetus. it is accepted that the abnormal accumulation of hydrophobic bile acids in maternal serum are responsible for the disease development . hydrophobic bile acids induce oxidative stress and apoptosis leading to the damage of the hepatic parenchyma and eventuauy extrahepatic tissues. as coenzyme q (coq) is considered an early marker of oxidative stress in this study, we sought to assess coq levels, bile acid profile and oxidative stress status in intrahepatic cholestasis.; Method CoQ, vitamin E and malondialdehyde were measured in plasma and/or tissues by HPLC-UV method whereas serum bile acids by capiuary electrophoresis in rats with ethinyl estradiol-induced cholestasis and women with pregnancy cholestasis; Results CoQ and vitamin E plasma levels were diminished in both rats and women with intrahepatic cholestasis. Furthermore, reduced CoQ was also found in muscle and brain of cholestatic rats but no changes were observed in heart or liver. In addition, a positive correlation between CoQ and ursodeoxycholic/lithocholic acid ratio was found in intrahepatic cholestasis suggesting that increased plasma lithocholic acid may be intimately related to CoQ depletion in blood and tissues;conclusion Significant CoQ and vitamin E depletion occur in bothanimals and humans with intrahepatic cholestasis likely as the result of increased hydrophobic bile acids known to produce significant oxidative stress. Present findings further suggest that antioxidant supplementation complementary to traditional treatment may improve cholestasis outcome.; ICP is characterized by the accumulation of bile acids (BA) in maternal serum which are likely responsible for the development of the disease because of their