紫杉醇减少脊髓损伤后疤痕形成并促进突再生 ppt课件.pptxVIP

Title：Microtubule Stabilization Reduces Scarring and Causes AxonRegeneration After Spinal Cord Injury Farida Hellal et al. Science 331, 928 (2011); DOI: 10.1126/science.1201148;Backgrounds;Assumptions;Methods;;Figure S1: Taxol decreases fibronectin, NG2 proteoglycan and collagen IVaccumulation induced by spinal cord injury (A)Representation of lesioned spinal cord (box). (B-D) Mid-saggital sections of lesion site from rat treated with Vehicle or Taxol at 7 days post-injury. Taxol decreases ECM deposit in the core of the lesion as assessed by fibronectin (B), collagen IV (C) and NG2 proteoglycan (D) immunostainings. The dashed lines outline the lesion site. Scale bar, 150 μm.;;Figure S3: Taxol does not induce apoptosis in the injured spinal cord (A) Representation of lesioned spinal cord (box). (B-C) Mid-saggital sections of lesion site from rats treated with Vehicle or Taxol stained with TUNEL at 3 (B) and 7 (C) days after dorsal hemisection injury. Scale bar, 150 μm. (D) Taxol does not affect the cell death induced by spinal cord injury. Results expressed as mean ± SEM; 3dpi: p= 0.628;7dpi: p= 0.679; two-tailed t test (n= 7 animals per group);Conclusion 1:;2. Then , they examined whether stabilizing the microtubule network hinders TGF- β signaling and attenuates fibrogenesis .;Fig. 2. Taxol dampens TGF-β signaling. (A) Taxol treatment increases total tubulin and decreases tyrosinated tubulin in the lesion site，enabling kinesin-1 to bind tightly to microtubules (15) .(B) Kinesin-1 enrichment in microtubule fraction of Taxol-treated lesion site. (C) His-Smad2 binds to kinesin-1 and endogenous Smad2 coimmunoprecipitates with kinesin-1 (D) of brain and spinal cord extracts. ;Fig. 2. Taxol dampens TGF-β signaling.  (E and F) Taxol alters microtubule-based cargo transport. (E) Overlay of colorcoded time series of red fluorescent protein (RFP)–labeled peroxisomes bound to the kinesin-1 (KIF5) motor domain upon Rapalog addition. Blue marks the initial distri