tirapmal tlr-4基因多态性对儿童脓毒症的发病和外周血中tlr4 tirap水平影响-effect of tiramal tlr - 4 gene polymorphism on the incidence of sepsis and tl r4 tirap level in peripheral blood of children.docx

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tirapmal tlr-4基因多态性对儿童脓毒症的发病和外周血中tlr4 tirap水平影响-effect of tiramal tlr - 4 gene polymorphism on the incidence of sepsis and tl r4 tirap level in peripheral blood of children.docx

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tirapmal tlr-4基因多态性对儿童脓毒症的发病和外周血中tlr4 tirap水平影响-effect of tiramal tlr - 4 gene polymorphism on the incidence of sepsis and tl r4 tirap level in peripheral blood of children

目录中文摘要···························································································· （1）英文摘要···························································································· （2）论文题目···························································································· （4）前言·························································································· （4）材料与方法·················································································· （5）结果··························································································· （13）讨论··························································································· （19）结论··························································································· （23）参考文献····················································································· （23）文献综述···························································································· （27）参考文献····················································································· （34）附件·································································································· （38）缩略语表···························································································· （41）攻读学位期间发表论文情况··································································· （42）个人简历···························································································· （43）致谢·································································································· （44）中文摘要目的（1）探讨 TOLL 样受体 4（TLR4）基因 Asp299Gly、Thr399Ile、位点及 TOLL/白介素-1 受体相关蛋白/MyD88 连接蛋白（TIRAP/Mal）基因 Ser180Leu 位点多态性与脓毒症的易感性关系；（2）探讨上述基因多态性对其相应蛋白 TLR4、TIRAP 表达水平的影响；（3）外周血中 TLR4、TRIAP 水平与脓毒症严重程度的相关性。方法对 20 例正常儿童及 60 例脓毒症患儿静脉采血提取全血 DNA 后，使用聚合酶链式反应---限制性片段长度多态性（PCR-RFLP）技术对 TIRAP/Mal 基因 Ser180Leu 及 TLR4 基因 Asp299Gly、Thr399Ile 位点基因多态性进行检测分析；同时应用酶联免疫吸附试验（ELISA）检测各样本中血清总 TLR4、TIRAP 浓度，且对 60 例脓毒症患儿应用小儿危重病例评分方法(PCIS)将脓毒症