Survivin mRNA在肾透明细胞癌组织和外周血中表达研究.docVIP

Survivin mRNA在肾透明细胞癌组织和外周血中表达的研究 【摘要】 目的 探讨Survivin mRNA在肾透明细胞癌(renal clear cell carcinoma，RCCC)患者组织和外周血中的表达及其意义。方法 采用逆转录聚合酶链反应（RTPCR）法检测46例RCCC患者肿瘤组织和15例健康志愿者外周血细胞中Survivin mRNA表达情况。结果 46例RCCC患者中有21例外周血细胞Survivin mRNA阳性表达，表达率为45.7％。对照组15例中有2例Survivin mRNA阳性表达，阳性率为13.3％，RCCC组Survivin mRNA阳性率明显高于对照组（P＜0.05）。46例RCCC患者同时收集到肿瘤组织和外周血，Survivin mRNA阳性表达分别为63.0%和45.7%，两者具有相关性（P＜0.01）。结论 Survivin mRNA在RCCC肿瘤组织中有显著的阳性表达，应用RTPCR法检测外周血细胞Survivin mRNA的表达，诊断RCCC敏感性、特异性高，且创伤性小，因此Survivin mRNA有可能成为RCCC诊断的一个新的敏感指标，是目前进行肿瘤早诊的理想检测方法之一。 【关键词】 肾透明细胞癌 Survivin 逆转录聚合酶链反应 外周血 【Abstract】 Objective To study the expression and significance of Survivin mRNA in tumoral tissues and blood cells of patients with renal clear cell carcinoma（RCCC）.Methods Survivin mRNA in tumoral tissues of 46 patients with RCCC, 15 normal renal tissues, blood cells of 46 patients with RCCC, and 15 healthy volunteers were detected. Survivin and βactin were amplified by using RTPCR.Results 21 cases (45.7%) showed positive Survivin mRNA signals in blood in patients with RCCC. Only 2 patiemts (13.3%) in control group showed positive Survivin mRNA signals in blood. The expression of Survivin mRNA difference between experimental group and control group was significant (P＜0.05).The positive rates of Survivin mRNA in tumor tissue and blood were 63.0% and 45.7% ,respectively (P＜0.01).Conclusion Carcinoma tissues of the patients with RCCC showed significantly positive expression of Survivin mRNA. High sensitivity and the specificity of expression of blood Survivin mRNA to provide a simple, microinvasive diagnostic test to identify patients with RCCC .RTPCR was an effective method for detection of Survivin mRNA in the blood of patients with RCCC. 【Key words】 renal clear cell carcinoma, Survivin,RTPCR,peripheral blood 肾细胞癌（renal cell carcinoma，RCC）是泌尿生殖系统常见的恶性肿瘤，占泌尿外科肿瘤的第二位，其患病率占全身恶性肿瘤的3%［1］。根据病理分类，肾细胞癌又分为透明细胞癌、乳头状癌、颗粒细胞癌及未分化癌等几种类型，其中大多数为透明细胞癌(renal clear cell carcinoma，RCCC)，约占总数的67.6%～76.2%［1］。目前对于肾癌尚无特殊有效的治疗方法，