久泻灵冲剂对溃疡性结肠炎大鼠结肠病变氧化损伤影响.docVIP

久泻灵冲剂对溃疡性结肠炎大鼠结肠病变氧化损伤的影响 作者：李海龙,吴玉泓,楚惠媛,车敏,安方玉,王宏伟 【摘要】 目的 观察久泻灵冲剂对溃疡性结肠炎模型大鼠的影响,探讨其作用机制。方法 采用异种异体结肠黏膜组织致敏法和乙酸局部灌肠相结合的方法制造动物模型。将Wistar大鼠随机分为空白对照组、模型组、阳性对照组及久泻灵冲剂大、中、小剂量组。连续给药14 d,末次给药后24 h处死大鼠,制备结肠组织匀浆,生化法检测结肠组织中谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、超氧化物歧化酶(SOD)、一氧化氮(NO)、总一氧化氮合酶(tNOS)和诱导型一氧化氮合酶(iNOS)的水平。结果 久泻灵冲剂可明显降低结肠组织中NO、tNOS、iNOS和MDA的活性或水平,提高GSH-Px、SOD的活性。结论 久泻灵冲剂对溃疡性结肠炎模型大鼠有显著的干预作用,其机制可能与抗氧自由基损伤,抑制NO、tNOS和iNOS生成,增强抗氧化酶活性有关。 【关键词】 久泻灵冲剂；溃疡性结肠炎；谷胱甘肽过氧化物酶；丙二醛；超氧化物歧化酶；一氧化氮；一氧化氮合酶；大鼠 Abstract：Objective To observe the effects of Jiuxieling granules on rats with ulcerative colitis and explore its mechanism. Methods Ulcerative colitis models were established by immune method combined with local stimulation of 5% acetic acid. Wistar rats were randomly divided into control, model, positive control and low, middle, high dose Jiuxieling granules group. Rats in positive control group were treated with sulfasalazine, and those in three treatment groups were treated with low, middle and high dosage of Jiuxieling granules respectively for 14 days continuously. 24 hours after last administration, the rats were killed to obtain colon mucous membrane to test GSH-Px, SOD, MDA, NO, tNOS and iNOS concentration of the homogenate of colon mucous membrane. Results Jiuxieling granules could markedly improve the levels of GSH-Px and SOD activity, whereas decrease the levels of MDA, NO, TNOS and iNOS. Conclusion Jiuxieling granules exert its marked curative effects on rats of ulcerative colonitis by resisting oxygen free radical lesion, inhibiting the generation of NO and NOS, and enhancing the activity of antioxidase. Key words：Jiuxieling granules；ulcerative colitis；GSH-Px；MDA；SOD；NO；NOS；rat 　　溃疡性结肠炎(ulcerative colitis,UC)是以腹泻、黏液脓血便、腹痛为主要临床症状,以结肠黏膜慢性炎症和溃疡形成为病理特点的一种慢性炎症性肠病。该病治愈难度大,且愈后又常易复发。近年来发现自由基的产生及脂质过氧化物反应增强与UC损伤的病理改变关系密切[1]。本研究通过观察UC急慢性病变复合模型大鼠结肠组织匀浆中谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、超氧化物歧化酶(SOD)、一氧化氮(NO)、总一氧化氮合酶(tNOS)和诱导型一氧化氮合酶(iNOS)的变化及久泻灵冲剂对其的影响,旨在进一步了解其治疗UC的作用机制,为临床用药奠定实验基础。 　　1 材料与方法 　　1.1