AQP1和VEGF在大鼠视网膜缺血再灌注损伤中表达.docVIP

AQP1和VEGF在大鼠视网膜缺血再灌注损伤中的表达 作者：张金红，张文芳，鲁建华，张书，梁丽 【摘要】 　　目的：研究大鼠视网膜缺血再灌注损伤后水通道蛋白1和血管内皮生长因子的表达情况并探讨二者在视网膜缺血再灌注损伤中表达的意义。方法：通过提高眼内压的方法建立大鼠视网膜缺血再灌注模型，于术后6，12，24，48h；3，7d用免疫组化的方法检测水通道蛋白1和血管内皮生长因子在大鼠视网膜的表达情况，并以正常大鼠视网膜作为对照。结果：正常对照组大鼠视网膜未能检测到血管内皮生长因子的阳性表达，缺血再灌注12h后开始出现表达，48h达高峰，且差异具有统计学意义（Plt;0.01）。水通道蛋白1在正常对照组可见到阳性表达，缺血再灌注组随时间增长表达不断增强，7d在外层视网膜也出现表达，差异有统计学意义（Plt;0.01）。结论：水通道蛋白1和血管内皮生长因子在视网膜缺血性疾病中起着重要的作用，二者可能共同影响了视网膜的水代谢过程。 【关键词】 水通道蛋白1；血管内皮生长因子；缺血再灌注；大鼠 　　Abstract　AIM:To investigate the expression levels and significance of aquaporin1 (AQP1) and vascular endothelial growth factor(VEGF) in the retinal ischemia reperfusion injury(RIRI). METHODS:The model of transient RIRI of the rat retina was constructed by elevation of the intraocular pressure .The expression levels of AQP1 and VEGF was measured 6, 12, 24 and 48 hours, 3, 7 days after retinal ischemia using immunohistochemical staining. Normal retina was treated as control group. RESULTS: VEGF positive cells were not found in normal group and began to express after 12 hours in ischemia group, increased gradually and reached maximal levels 48 hours after RIRI. There was statistical difference between 48 hours group and other ischemia reperfusion group (Plt;0.01). AQP1 positive cells were found in the outer retina in normal group, increased gradually in ischemia reperfusion group and also were found in the inner retina 7 days after RIRI, and the difference was significant (Plt;0.01). CONCLUSION: AQP1 and VEGF may play an important role in various blinding ischemic ocular diseases, and coeffect the water metabolism of retina.  　　KEYWORDS: AQP1; VEGF; ischemia reperfusion; rat 　　0　引言 　　视网膜血管性疾病包括视网膜中央动脉/分支动脉阻塞，静脉阻塞，早产儿视网膜病变，糖尿病性视网膜病变等，以及临床常见的闭角型青光眼急性发作和一些影响视网膜血流的内眼手术，这些疾病和手术均存在着不同程度的视网膜缺血再灌注损伤（retinal ischemia reperfusion injury，RIRI），其造成视网膜的水肿是影响患者视力恢复的主要因素之一。水通道蛋白1（aquaporin1，AQP1）作为一种调节水分子跨膜转运的膜蛋白，维持体内水的渗透平衡[1]。其异常表达，打破了视网膜组织对水的调解平衡，并在缺血缺氧的过程中诱导新生血管的生成，导致了视网膜的水肿[2,3]。血管内皮生长因子（vascular endothelial gro