三七总皂甙对酒精诱导兔骨髓基质干细胞成脂基因422(ap2)mrna表达的影响-中医骨伤科学专业论文.docxVIP

3 3 PNS on alcohol-induced bone marrow stromal cells into fat cell specific gene 422(aP2)mRNA expression in the pilot study ABSTRACT Objective: PNS on whether alcohol-induced inhibition of bone marrow stromal stem cells (BMSCs) adipogenic differentiation, and its inhibitory effect and dose relationship. Methods: The femur bone marrow fluid from 4-week-old New Zealand white rabbits, with density gradient centrifugation in vitro separation BMSCs, conduct training, passage, with inverted phase of the cell were observed patterns of growth. Will be conveyed to the second generation cell-culture plate 6, were randomly divided into control group, model, high-dose treatment group and low-dose treatment groups, each with 10 boards. Blank group, in every medium 1ml saline added 50μl; model group, in 1ml of alcohol added to the culture medium (99.8% ethanol) 50μl; high and low dose treatment group, 1ml in the medium to add alcohol-induced 50μl Adipogenic differentiation success, and then joined PNS injection 100μl, 50μl. Four groups of cells in drug use before and after the intervention difference between inverted microscope patterns change, induced in the first eight days, each for 10-cell line oil red O staining fat cell count, triglyceride determination, alkaline phosphate Determination of activity, Intervention in the treatment of the first 15 days, each for 10-cell lines respectively after the PAGE PAGE 10 oil red O staining fat cell count, triglyceride determination, the activity of ALP, and fat-related gene 422(aP2)mRNA expression Testing. Results: Cultured cells in inverted phase under the microscope observation, the basic shape of a spindle, the colony was shape of a chrysanthemum by the centre to around scattering, in line with the growth of BMSCs and characteristics; Induction in the first eight days, blank group oil red O staining fat cell count, triglyceride levels were 10.42±5.23n/cm2, 1.16±0.01μg/hole, compared with the model group of 124.15±13.5